RE: Re - formaldehyde substitutes [Prefer]
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | histonet <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Wed, 28 Jun 2000, Petrilli, Michael wrote:
> We had problem with prefer-fixed tissue and immunos ... have
> discontinued use.
Can you tell us all what the problem was?
Although the exact composition of Anatech's "Prefer" fixative
is a trade secret, its active ingredient (glyoxal) and mode
of action (aldehyde reactivity similar to formaldehyde's) are
clearly explained in Anatech's literature (www.anatech.com)
and in some peer-reviewed publications (e.g. Dapson, 1993
Biotech Histochem 68:75-82). Glyoxal fixation has also been
explained in various HistoNet messages that should be findable
in the Archives.
Any problem encountered with a glyoxal-based fixative but not
with formaldehyde would be of general interest. So, for that
matter, would any applications for which glyoxal turns out
to give better fixation or antigen preservation.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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