RE: Lack of replies is a problem
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From: | "Terrett, Barb" <Barb.Terrett@uhn.on.ca> |
To: | 'Tim Morken' <timcdc@hotmail.com>, Histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain |
Ditto!! A number of times I have had questions answered before I even asked
them!!! Good service is hard to find :)
> -----Original Message-----
> From: Tim Morken [SMTP:timcdc@hotmail.com]
> Sent: July 5, 2000 9:58 AM
> To: Histonet@pathology.swmed.edu
> Subject: Lack of replies is a problem
>
> Greg wrote: <Hope all replies are posted for all to see!>
>
> Personal replies are a problem on this listserver. There have been
> several
> threads recently where the original questioner thanks "everyone" for their
>
> replies, yet I have seen few, if any, replies posted. That means we are
> all
> missing out on some good information.
>
> Please, all who use this listserver, don't ever think that your replies
> don't appeal to everyone else. If it is not truely personal in nature, and
>
> addresses the question, we would all like to see it! I am always amazed at
>
> the seeminly inconsequential tidbits that appear here and turn out to be
> enormously useful later on.
>
>
>
> Tim Morken, B.A., EMT(MSA), HTL(ASCP)
> Infectious Disease Pathology
> Centers for Disease Control
> MS-G32
> 1600 Clifton Rd.
> Atlanta, GA 30333
> USA
>
> email: tim9@cdc.gov
> timcdc@hotmail.com
>
> Phone: (404) 639-3964
> FAX: (404)639-3043
>
>
>
>
>
> ----Original Message Follows----
> From: Greg Dobbin <dobbin@Upei.CA>
> To: Lynn Gardner <lynn-gardner@uiowa.edu>, Histonet@pathology.swmed.edu
> Subject: Re: Insitu Paraffin
> Date: Tue, 04 Jul 2000 09:54:25 -0300 (ADT)
>
> I Lynn,
> I'm learning more and more about In situ Hyb. daily, so I am no
> expert by any stretch. I hadn't thought of the waterbath as a source
> of RNase contamination until you mentioned it, but is it possible that
> the RNA in the tissue is protected buy the hydrophobic nature of the
> paraffin?? If we can't find out for sure, I guess using DEPC treated
> water wouldn't be too much of a hardship! Looking forward to
> following this one up. Hope all replies are posted for all to see!
> Cheers! Greg
>
> Date sent: Thu, 29 Jun 2000 18:13:12 -0500
> From: Lynn Gardner <lynn-gardner@uiowa.edu>
> Subject: Insitu Paraffin
> Forwarded to: DOBBIN@acad1.cs.upei.ca
> To: Histonet@pathology.swmed.edu
>
> > Dear histonetters,
> >
> > I would be most interested in finding someone out there who does Insitu
>
> on
> > Paraffin sections routinely and who has it running consistently. I
> would
> > like to find out if that person or persons would be willing to share
> their
> > procedure with us as we would like to get it started here. We already
> do
> > Insitu on Frozens but would like to work with paraffin.
> >
> > Any help is greatly appreciated. Please either e-mail the information
> or
> > feel free to fax it to me at 319-335-7770.
> >
> > Thanks everyone!
> >
> > Sincerely,
> > Lynn Gardner, HT(ASCP)
> >
> >
>
>
> >
> >
> >
> >
> >
> >
> Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 Unviversity Ave.
> Charlottetown, P.E.I.
> Canada, C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851
>
>
>
>
>
> ________________________________________________________________________
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