RE: Lack of replies is a problem

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From:"Terrett, Barb" <Barb.Terrett@uhn.on.ca>
To:'Tim Morken' <timcdc@hotmail.com>, Histonet@pathology.swmed.edu
Reply-To:
Content-Type:text/plain

Ditto!! A number of times I have had questions answered before I even asked
them!!! Good service is hard to find  :)

> -----Original Message-----
> From:	Tim Morken [SMTP:timcdc@hotmail.com]
> Sent:	July 5, 2000 9:58 AM
> To:	Histonet@pathology.swmed.edu
> Subject:	Lack of replies is a problem
> 
> Greg wrote: <Hope all replies are posted for all to see!>
> 
> Personal replies are a problem on this listserver.  There have been
> several 
> threads recently where the original questioner thanks "everyone" for their
> 
> replies, yet I have seen few, if any, replies posted. That means we are
> all 
> missing out on some good information.
> 
> Please, all who use this listserver, don't ever think that your replies 
> don't appeal to everyone else. If it is not truely personal in nature, and
> 
> addresses the question, we would all like to see it! I am always amazed at
> 
> the seeminly inconsequential tidbits that appear here and turn out to be 
> enormously useful later on.
> 
> 
> 
> Tim Morken, B.A., EMT(MSA), HTL(ASCP)
> Infectious Disease Pathology
> Centers for Disease Control
> MS-G32
> 1600 Clifton Rd.
> Atlanta, GA 30333
> USA
> 
> email: tim9@cdc.gov
>        timcdc@hotmail.com
> 
> Phone: (404) 639-3964
> FAX:  (404)639-3043
> 
> 
> 
> 
> 
> ----Original Message Follows----
> From: Greg Dobbin <dobbin@Upei.CA>
> To: Lynn Gardner <lynn-gardner@uiowa.edu>, Histonet@pathology.swmed.edu
> Subject: Re: Insitu Paraffin
> Date: Tue, 04 Jul 2000 09:54:25 -0300 (ADT)
> 
> I Lynn,
> I'm learning more and more about In situ Hyb. daily, so I am no
> expert by any stretch. I hadn't thought of the waterbath as a source
> of RNase contamination until you mentioned it, but is it possible that
> the RNA in the tissue is protected buy the hydrophobic nature of the
> paraffin?? If we can't find out for sure, I guess using DEPC treated
> water wouldn't be too much of a hardship! Looking forward to
> following this one up. Hope all replies are posted for all to see!
> Cheers!  Greg
> 
> Date sent:      	Thu, 29 Jun 2000 18:13:12 -0500
> From:           	Lynn Gardner <lynn-gardner@uiowa.edu>
> Subject:        	Insitu Paraffin
> Forwarded to:   	DOBBIN@acad1.cs.upei.ca
> To:             	Histonet@pathology.swmed.edu
> 
>  > Dear histonetters,
>  >
>  > I would be most interested in finding someone out there who does Insitu
> 
> on
>  > Paraffin sections routinely and who has it running consistently. I
> would
>  > like to find out if that person or persons would be willing to share 
> their
>  > procedure with us as we would like to get it started here. We already
> do
>  > Insitu on Frozens but would like to work with paraffin.
>  >
>  > Any help is greatly appreciated. Please either e-mail the information
> or
>  > feel free to fax it to me at 319-335-7770.
>  >
>  > Thanks everyone!
>  >
>  > Sincerely,
>  > Lynn Gardner, HT(ASCP)
>  >
>  >
> 
> 
>  >
>  >
>  >
>  >
>  >
>  >
> Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 Unviversity Ave.
> Charlottetown, P.E.I.
> Canada,  C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851
> 
> 
> 
> 
> 
> ________________________________________________________________________
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> 



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