RE: Immuno EM methods

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From:jim <> (by way of histonet)
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Suggest that you go into the Links in our online and do a Control F to find
"gold". You will get several internet sites, several of these have great
protocols available.
Jim Darley
ProSciTech                 Microscopy PLUS
PO Box 111, Thuringowa  QLD  4817  Australia
Ph +61 7 4774 0370  Fax:+61 7 4789 2313
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560            

On Friday, July 07, 2000 9:04 AM, Joyce Kotzuk []
> My P.I. is interested in trying immunohistochemistry on tissue that has been
> prepared for EM, even tissue that is already embedded in plastic or resin or
> whatever.  I have never done EM, nor worked with (ie. cut) tissue in plastic.
>  Could someone suggest some references or a protocol for doing immuno on
> tissue such as this?  I got the references about antigen retrieval in EM
> tissue that were recently posted on the Histonet, but I need basic methods,
> and how they differ from regular immuno. Thanks in advance,
> Joyce Kotzuk, Univ. of New Mexico pathology dept.

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