RE: Immuno EM methods
<< Previous Message | Next Message >>
From: | jim <jim@proscitech.com.au> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Suggest that you go into the Links in our online and do a Control F to find
"gold". You will get several internet sites, several of these have great
protocols available.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service@proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com
On Friday, July 07, 2000 9:04 AM, Joyce Kotzuk [SMTP:JKotzuk@salud.unm.edu]
wrote:
> My P.I. is interested in trying immunohistochemistry on tissue that has been
> prepared for EM, even tissue that is already embedded in plastic or resin or
> whatever. I have never done EM, nor worked with (ie. cut) tissue in plastic.
> Could someone suggest some references or a protocol for doing immuno on
> tissue such as this? I got the references about antigen retrieval in EM
> tissue that were recently posted on the Histonet, but I need basic methods,
> and how they differ from regular immuno. Thanks in advance,
> Joyce Kotzuk, Univ. of New Mexico pathology dept.
> jkotzuk@salud.unm.edu
>
<< Previous Message | Next Message >>