-----Original Message-----
		From:	HistoNet Server
[mailto:histonet@pathology.swmed.edu]
		Sent:	Wednesday, July 12, 2000 4:01 AM
		To:	HistoNet Server
		Subject:	Daily Digest


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 05:45:53 -0500
		From: Lee & Peggy Wenk <lpwenk@mail.netquest.com>
		Subject: Re: Fwd: NSH Scholarships and Starting a Histology
program

		This is Peggy Wenk. I am the program director
		of a HT and a HTL program at a hospital, and
		I am giving a workshop at NSH on how to set 
		up a HT or HT school.

		If anyone is interested in how to start a program, 
		please give me a call at 248/551-9079. (work)

		PLEASE, only call me if you are REALLY interested
		in starting a program. If you have some hospitals
		and/or colleges SERIOUSLY interested in starting
		a program. We need to talk for a LONG time, 
		and probably several times. But I can
		give you some ideas on how to start. I have helped
		other hospitals and colleges with ideas, and
		try to come up with ideas that would work given
		the hospitals and/or colleges involved (there is
		no RIGHT way to set up a program. There are, on
		the other hand, wrong ways.)

		If you simply think a school is a good idea, or
		something you can do in your "spare time" at 
		work, or because you have always wanted to teach, 
		or are just curious, please don't call. I don't
		mean to sound rude, but these conversations do
		take up a lot of my time (read hours), and as you 
		know, time is limited to everyone who works in a 
		hospital.

		So, yes, I'm willing to to talk, but PLEASE, 
		don't all 1200+ histonetters try calling me
		on this topic.

		I have thought about giving this as a teleconference,
		but am concerned that this is a really limited
		topic with a very small audience. Maybe those who
		are just curious, etc., could let me know if this
		is a teleconference you would like to hear. Respond
		via histonet or through my home email which is:

		Lpwenk@netquest.com


		Peggy A. Wenk, HTL(ASCP)
		Schools of HIstotechnology
		William Beaumont Hospital
		Royal Oak, MI 48073



		DENISE BLAND-PIONTEK wrote:
		> 
		> Subject: NSH Scholarships and Starting a Histology program
		> Date: Mon, 10 Jul 2000 18:01:29 -0600
		> From: "DENISE BLAND-PIONTEK" <DBLANDP1@FAIRVIEW.ORG>
		> To: <histonet@pathology.swmed.edu'>
		> 
		> I'm asking these questions for a fellow histotechnologist,
Pam Buzzard, who
		resides in Georgia. She will be joining the Histonet soon.
Does the NSH offer
		any scholarships for attending nationals? Does anyone have a
suggested
		curriculum with what each course should entail as far as
starting a histotech
		program is concerned?  She has been asked by a local college
to initiate a
		program, but has little info to go on. I have already
suggested attending the
		workshop that is going to be offered at Nationals this year.
I will inform her
		of all answers posted on the histonet, but she can otherwise
be contacted at
		PDBUZZ68@aol.com.
		> Thank you,
		> Denise Bland-Piontek HTL (ASCP)
		> Fairview University Medical Center
		> Minneapolis, MN


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 05:46:08 -0500
		From: Lee & Peggy Wenk <lpwenk@mail.netquest.com>
		Subject: how to unsubscribe for vacations

		For those going on vacations, remember to
		unsubscribe from histonet. Please don't set
		your system to tell us you are on vacation.
		At fifty messages a day, that can be
		overwhelming to histonet.

		Print this page out and save it. Or save
		this page as a file on your computer 
		until you need it.


		So, to unsubscribe:

		1. Address email to histonet

		histonet@pathology.swmed.edu


		Do NOT piggy-back this on someone else's
		message. It comes through with a "re:",
		or a "forward", and you will not be
		able to unsubscribe that way.


		2. In the subject area, write

		unsubscribe


		Please note that there are 11 letters.
		Make certain they are in the correct order.

		Do NOT add any other words, like "please"
		or "re:" or any quotation marks ("), or
		any exclamation marks (!).


		3. Hit Send or Transmit.


		That should do it. 


		When you get back from vacation, and
		want to rejoin Histonet, 

		1. Address an email to histonet at

		histonet@pathology.swmed.edu


		2. In the SUBJECT area, type

		subscribe


		again, no other words, no ",
		9 letters in the right order, etc.


		3. Hit send/transmit

		You should be rejoined in a
		few minutes to a few hours. Be
		patient. If you haven't been rejoined
		to histonet by the next day, try again.


		Have fun and see you when you get back.

		Peggy A. Wenk, HTL(ASCP)
		William Beaumont Hospital
		Royal Oak, MI 48073


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 07:44:18 -0500
		From: "Coskran, Timothy M"
<timothy_m_coskran@groton.pfizer.com>
		Subject: IHC on Liver

		As many of the replies indicate, the problem could be due to
endogenous
		biotin as well as endogenous peroxidase.  We've found that
using one of the
		available avidin-biotin blocking kits will help reduce the
background.  You
		could also change your detection from a standard ABC or LSAB
to the Envision
		technique which avoid the use of biotin.  We've used this
kit with a few
		antibodies on liver sections with good success.

		Good luck,


		Tim Coskran
		Pfizer


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 08:12:17 -0500
		From: "Tom T. McNemar" <TMcNemar@lmhealth.org>
		Subject: H. Pylori by IHC...

		Does anyone have a good protocol for H. Pylori on the
Ventana ES?  I have
		tried a couple of different antibodies. Thanks for your
help.

		Tom Mc Nemar
		Pathology Supervisor
		Licking Memorial Hospital
		Newark, Ohio
		I USE CELL MARQUE AB. THE PROTOCOL ON THE ES IS: ANTIGEN
RET. 25MINS IN CITRATE BUFFER, COOL 10 MINS, 32MIN INCUBATION AND AMPLIFY


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 08:12:32 -0500
		From: Jerry Chi-Yuan Hu <ragnarok@ruf.rice.edu>
		Subject: Slightly OT: Live cells anyone?


		Hello Everyone,

		I am looking for a way to isolate cells from thin slices (at
100 um 
		thick) and to start a cell line from these cells.  Everyone
I've spoken to 
		says that the fresh tissue has to be embedded for slicing in
a microtome 
		cryostat, though slices of 100 um might crack...  I also do
not know if 
		the embedding medium is going to kill my cells.  

		If anyone knows how to use the microtome (or cryotome) to
get thick slices
		of live cells please let me know.  Your help is greatly
appreciated!

		Jerry Hu



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 08:12:53 -0500
		From: dspjhd@cha.quebec.qc.ca
		Subject: Please unsubscribe





	
----------------------------------------------------------------------

		Date: 11 Jul 2000 08:13:07 -0500
		From: dspjhd@cha.quebec.qc.ca
		Subject: Please unsubscribe





	
----------------------------------------------------------------------

		Date: 11 Jul 2000 08:13:32 -0500
		From: Jenny Molde <ctsmolde@samiot.uct.ac.za>
		Subject: Marker for T-cells in sheep

		Dear Histonetters
		I am looking for a lymphocyte marker for sheep. Anyone out
there had any
		experience with this. Many thanks.

		Jenny Molde




	
----------------------------------------------------------------------

		Date: 11 Jul 2000 10:30:28 -0500
		From: "Vicki Gauch" <GauchV@mail.amc.edu>
		Subject: Re: consult charges, documentation

		Kathy,
		 We also had this dilemma....we changed our procedure so
that now when the
		physician calls to have a consult sent we fax him/her a
request form that has
		a portion stating that he is requesting us to send slides to
(the name of the
		physician we are sending to).  The physician signs this form
and faxes it back
		to us.  We process the request, send the slides on their way
and we keep those
		request forms on file.  We used to accept a phone request
but now we require
		that form to be filled out before we will even begin the
process.  Hope this
		helps...

		Have a great day  : )

		Vicki
		Albany Medical Center

		>>> hkcormier <hkcorm@banet.net> 07/10/00 07:13PM >>>
		Hello All,
		I have an interesting question for you all, our corporate
compliance
		program is currently looking at path consult charges...how
does everyone
		(anyone) document physician requests for consults. By this I
mean the
		patient's doctor calls the office and requests that the case
be sent out
		to another pathologist, not the "house" pathologist sending
our for a
		second opinion. Our issue is that we are a fairly small
hospital  with
		only 2 pathologist, our pathologists will routinely send out
cases for a
		second opinion, on say bone marrows to the regions top bone
marrow guy.
		The local visiting oncologist and ocasionally a local
surgeon - primary
		care physician will request that a case be sent out, some
where else.
		The issue is, is that it is usually a phone call from the
doctor or
		doctor's staff and we never receive any written order or
request. This
		our course would slow us all down and wreck havoc with our
customer
		service policy. Our corporate people feel that without
documentation
		from somewhere, this may be construed as fraud.  Any ideas?
Any one ever
		deal with this before? Any suggestions? Thanks!  Kathy





	
----------------------------------------------------------------------

		Date: 11 Jul 2000 10:31:05 -0500
		From: "MacDonald, Jennifer" <jmacdonald@sach.org>
		Subject: RE: consult charges, documentation

		The order can be faxed to you.  Attach the faxed order to
the report that
		you keep filed. This way you have official documentation of
the request.
		Tests can be initiated by a verbal order but must have a
written order on
		file.  Who pays for the consultation?

		Jennifer MacDonald

		> ----------
		> From: 	hkcormier[SMTP:hkcorm@banet.net]
		> Sent: 	Monday, July 10, 2000 6:13 PM
		> To: 	histonet@pathology.swmed.edu
		> Subject: 	consult charges, documentation
		> 
		> Hello All,
		> I have an interesting question for you all, our corporate
compliance
		> program is currently looking at path consult charges...how
does everyone
		> (anyone) document physician requests for consults. By this
I mean the
		> patient's doctor calls the office and requests that the
case be sent out
		> to another pathologist, not the "house" pathologist
sending our for a
		> second opinion. Our issue is that we are a fairly small
hospital  with
		> only 2 pathologist, our pathologists will routinely send
out cases for a
		> second opinion, on say bone marrows to the regions top
bone marrow guy.
		> The local visiting oncologist and ocasionally a local
surgeon - primary
		> care physician will request that a case be sent out, some
where else.
		> The issue is, is that it is usually a phone call from the
doctor or
		> doctor's staff and we never receive any written order or
request. This
		> our course would slow us all down and wreck havoc with our
customer
		> service policy. Our corporate people feel that without
documentation
		> from somewhere, this may be construed as fraud.  Any
ideas? Any one ever
		> deal with this before? Any suggestions? Thanks!  Kathy
		> 
		> 


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 10:31:19 -0500
		From: "Tom T. McNemar" <TMcNemar@lmhealth.org>
		Subject: RE: consult charges, documentation

		Our path secretary handles all of the consult sendouts.  We
never receive a
		written request either and I suspect that it would do us no
good to request
		one.  The secretary maintains a log of what was sent, where
it was sent,
		when, and who requested that it be sent.  When they are
returned, she then
		goes to her log and enters the date returned.

		It has never been an issue here..... maybe no one thought of
it..... maybe
		no one cares.......
		I'll be watching the replies.

		Tom Mc Nemar
		Pathology Supervisor
		Licking Memorial Hospital
		Newark, Ohio

		> -----Original Message-----
		> From:	hkcormier [SMTP:hkcorm@banet.net]
		> Sent:	Monday, July 10, 2000 7:14 PM
		> To:	histonet@pathology.swmed.edu
		> Subject:	consult charges, documentation
		> 
		> Hello All,
		> I have an interesting question for you all, our corporate
compliance
		> program is currently looking at path consult charges...how
does everyone
		> (anyone) document physician requests for consults. By this
I mean the
		> patient's doctor calls the office and requests that the
case be sent out
		> to another pathologist, not the "house" pathologist
sending our for a
		> second opinion. Our issue is that we are a fairly small
hospital  with
		> only 2 pathologist, our pathologists will routinely send
out cases for a
		> second opinion, on say bone marrows to the regions top
bone marrow guy.
		> The local visiting oncologist and ocasionally a local
surgeon - primary
		> care physician will request that a case be sent out, some
where else.
		> The issue is, is that it is usually a phone call from the
doctor or
		> doctor's staff and we never receive any written order or
request. This
		> our course would slow us all down and wreck havoc with our
customer
		> service policy. Our corporate people feel that without
documentation
		> from somewhere, this may be construed as fraud.  Any
ideas? Any one ever
		> deal with this before? Any suggestions? Thanks!  Kathy
		> 


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 10:31:53 -0500
		From: Cynthia Favara <cfavara@niaid.nih.gov>
		Subject: RE: Marker for T-cells in sheep

		Try contacting Pam Dilbeck at WSU. I have not done t-cel
markers in sheep,
		but have done in mice with success using Dako CD3 in murine
models. If you
		would like more info let me know.
		Cynthia Favara 
		NIAID/RML/LPVD 
		903 South 4th Street 
		Hamilton, MT 59840 
		PH: 406-363-9317 
		FAX: 406-363-9286 

		e-mail: CF98d@nih.gov <mailto:CF98d@nih.gov> 

		 



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 10:32:15 -0500
		From: "Paul Howard Lockwood" <TigrSnke@ix.netcom.com>
		Subject: Re: Mental illness: the other side

		- -----BEGIN PGP SIGNED MESSAGE-----
		Hash: SHA1

		Dear Bryan,
		     My thoughts exactly. I appreciate your courage in
voicing them.
		     As for the comments made about a supervisor needing to
know the
		mental state of their employees, I ask this question: At
what point
		does caring for an employee become intrusive, and even
discriminatory?
		A short seminar, or even a couple of semesters of Behavioral
		Psychology does not qualify someone to make a diagnosis, or
any
		judgement concerning a person's mental state. This is always
best left
		to a professional with years of training, and an internship
under
		their belt.
		     Sincerely,
		      Paul Howard Lockwood 
		- - -----Original Message-----
		From: Bryan Llewellyn <bryand@netbistro.com>
		To: Histonet <histonet@pathology.swmed.edu>
		Date: Monday, July 10, 2000 1:00 AM
		Subject: Mental illness: the other side


		>> Lastly, if we screen out all of the mentally ill from
jobs, what
		>> are we to do with them? They have rights, and one of them
is the
		right
		>> to pursue happiness by being gainfully employed.
		>
		>I too thought long and hard about whether I should reply to
the
		postings on
		>this subject.  I finally decided that I should because I
can bring an
		aspect
		>to the discussion that has not yet been made.  Please
excuse what may
		appear
		>to be bragging, but I am going to speak very frankly
because I have
		found
		>some off the comments made on this subject quite demeaning
and
		insulting.  I
		>do not apologise for saying this.  I think it needs to be
said.
		>
		>I am a long time chronic depressive.  I have been depressed
on and
		off
		>(mostly on) for about 50 years (I am 57), and have been a
passive
		suicide
		>since I was about eight.  The source of my emotional
problems is
		abuse as a
		>child; physical, sexual and emotional.  Did you ever wonder
where
		abused
		>children went when they grew up?  We hide among you.
		>
		>Depression is considered to be a mental illness.  I am
therefore one
		of
		>those people you are all wondering whether you should fire
because
		you are
		>concerned I might attack you.
		>
		>I do my job, and rather well, I always thought.  I am the
supervisor
		of a
		>small histology lab, and have been the supervisor of a very
large
		histology
		>lab years ago in Winnipeg.  I qualified at the highest
possible
		levels
		>available at the time (1969 and 1979) in two countries
(Britain and
		Canada).
		>I authored and administered for several years three
separate
		correspondence
		>courses for the Canadian professional Society in advanced
		histological
		>techniques.  I have written a few papers on histological
subjects
		(amyloid
		>staining, H&E substitutes).  I set up the StainsFile web
page, and
		have done
		>all the work for it with the exception of one article.  I
venture to
		suggest
		>that I have received considerable respect for my technical
abilities
		over
		>the years from coworkers and pathologists, some of whom
have been
		quite
		>demanding.
		>
		>I am politically active and have been asked on more than
one occasion
		to run
		>for office provincially.  I have been declared an honorary
woman
		because of
		>my strong committment to gender equity.  I feel just as
strongly
		about
		>racial equality and gay and lesbian issues.  I venture to
suggest
		that I
		>have made a noticeable contribution to society.
		>
		>I have been married to Linda for 37 years.  She has borne
the brunt
		of my
		>depression for all that time.  We have three children, all
of whom
		are well
		>educated (Jason a city planner, Stevyn a pharmacist, and
David a
		Ph.D.
		>student in organo-metallic chemistry at McGill
unioversity).  None of
		them
		>have inherited my depression.  All of them love and respect
me and
		will miss
		>me when I die.
		>
		>Mentall illness is no more a single entity than any other
branch of
		human
		>activity.  Both smallpox and the common cold are viral
diseases, but
		why
		>would anyone in their right mind think that a person with a
cold is
		as
		>dangerous as a person with smallpox.  Please get a
perspective. 
		Mental
		>illness ranges from the inocuous to the dangerous.  Most
people alive
		(that
		>means you) have some kind of a neurosis, and all neuroses
are a form
		of
		>mental illness.  From very personal experience, I can tell
you that
		those
		>who suffer most from mentall illnesses are the people with
it and
		those who
		>love them.  An example is the poor progress of the
StainsFile web
		site, on
		>which I have been unable to work for a year.
		>
		>I am most certainly not ashamed of my mental state.  In
fact, I am
		quite
		>proud of the fact that I have been strong willed enough to
rise above
		my
		>handicap.  I think I have made a contribution to society.
I have
		never hit
		>anyone.  I have never killed anyone.  I treat others with
respect. 
		Why
		>should I be discriminated against because someone else has
a hangup
		(a
		>neurosis) about emotional disorders?  Why should I be fired
when it
		is your
		>problem?  Would you fire me if I had one arm?  Would you
refuse to
		work with
		>me if I stuttered?  Would you send me to the back of the
bus if I
		were
		>black?  Please tell me how discriminating against me
because of my
		>depression is any different from those things.
		>
		>Remember, we are all innocent until proven guilty.  Please
do not
		fire me
		>until I have done something worth being fired for.  As for
not hiring
		>someone because in a ten minute interview, an interviewer
with no
		>psychiatric training decides the candidate is nuts, please,
get a
		life!
		>
		>I would request that people think before they post comments
that are
		>demeaning to those like me.
		>
		>Bryan Llewellyn
		>Depressive, and absolutely NOT ashamed of it.
		>
		>
		>
		>
		>
		>
		>
		>
		>
		>
		>
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----------------------------------------------------------------------

		Date: 11 Jul 2000 10:32:41 -0500
		From: jim <jim@proscitech.com.au>
		Subject: RE: Slightly OT: Live cells anyone?

		Easy. You need a Vibratome. These have a vibrating blade and
this means
		cutting 
		can occur without compression. You can look at our online
pages, but we cannot

		sell into the N American market.
		Cheers
		Jim Darley

		ProSciTech                 Microscopy PLUS
		PO Box 111, Thuringowa  QLD  4817  Australia
		Ph +61 7 4774 0370  Fax:+61 7 4789 2313
service@proscitech.com
		Great microscopy catalogue, 500 Links, MSDS, User Notes
		ABN: 99 724 136 560                      www.proscitech.com

		On Tuesday, July 11, 2000 10:53 PM, Jerry Chi-Yuan Hu 
		[SMTP:ragnarok@ruf.rice.edu] wrote:
		>
		> Hello Everyone,
		>
		> I am looking for a way to isolate cells from thin slices
(at 100 um
		> thick) and to start a cell line from these cells.
Everyone I've spoken to
		> says that the fresh tissue has to be embedded for slicing
in a microtome
		> cryostat, though slices of 100 um might crack...  I also
do not know if
		> the embedding medium is going to kill my cells.
		>
		> If anyone knows how to use the microtome (or cryotome) to
get thick slices
		> of live cells please let me know.  Your help is greatly
appreciated!
		>
		> Jerry Hu
		>



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 10:33:20 -0500
		From: "Johnson, Mickey" <JohnsoM@shmc.org>
		Subject: RE: Mental illness: the other side

		Thank you all for your bravery! I salute you. Co-dependence
with a parent
		short of physical abuse only catches a thin edge of what
physically abused
		children experience. In addition, as is clear from this
thread of
		correspondence, the experience has life long consequences
for the abused,
		which never goes away. I am amazed and frankly disgusted
that our judicial
		system does not recognize this fact and still releases level
3 sexual
		offenders back to molest more children! We all need to apply
pressure on the
		legislative and judicial systems when the  opportunity
presents itself to
		change this.  Thanks for taking the time to read my
thoughts.
		Best Regards.

		Mickie

		- -----Original Message-----
		From: Thomas J. Kuwahara [mailto:tom@resolve3d.com]
		Sent: Monday, July 10, 2000 10:02 AM
		To: Bryan Llewellyn
		Cc: Histonet
		Subject: Re: Mental illness: the other side


		Dear Bryan:  I've been off a couple of weeks and it's been
quite a
		morning reading all my 300+ emails but I have to salute your
bravery in
		sending such a very personal and moving message over this
often
		contentious internet site.  If you ever want to come work
for a private
		company in San Francisco, let me know.  Regards, Tom

		Bryan Llewellyn wrote:
		> 
		> > Lastly, if we screen out all of the mentally ill from
jobs, what
		> > are we to do with them? They have rights, and one of
them is the right
		> > to pursue happiness by being gainfully employed.
		> 
		> I too thought long and hard about whether I should reply
to the postings
		on
		> this subject.  I finally decided that I should because I
can bring an
		aspect
		> to the discussion that has not yet been made.  Please
excuse what may
		appear
		> to be bragging, but I am going to speak very frankly
because I have found
		> some off the comments made on this subject quite demeaning
and insulting.
		I
		> do not apologise for saying this.  I think it needs to be
said.
		> 
		> I am a long time chronic depressive.  I have been
depressed on and off
		> (mostly on) for about 50 years (I am 57), and have been a
passive suicide
		> since I was about eight.  The source of my emotional
problems is abuse as
		a
		> child; physical, sexual and emotional.  Did you ever
wonder where abused
		> children went when they grew up?  We hide among you.
		> 
		> Depression is considered to be a mental illness.  I am
therefore one of
		> those people you are all wondering whether you should fire
because you are
		> concerned I might attack you.
		> 
		> I do my job, and rather well, I always thought.  I am the
supervisor of a
		> small histology lab, and have been the supervisor of a
very large
		histology
		> lab years ago in Winnipeg.  I qualified at the highest
possible levels
		> available at the time (1969 and 1979) in two countries
(Britain and
		Canada).
		> I authored and administered for several years three
separate
		correspondence
		> courses for the Canadian professional Society in advanced
histological
		> techniques.  I have written a few papers on histological
subjects (amyloid
		> staining, H&E substitutes).  I set up the StainsFile web
page, and have
		done
		> all the work for it with the exception of one article.  I
venture to
		suggest
		> that I have received considerable respect for my technical
abilities over
		> the years from coworkers and pathologists, some of whom
have been quite
		> demanding.
		> 
		> I am politically active and have been asked on more than
one occasion to
		run
		> for office provincially.  I have been declared an honorary
woman because
		of
		> my strong committment to gender equity.  I feel just as
strongly about
		> racial equality and gay and lesbian issues.  I venture to
suggest that I
		> have made a noticeable contribution to society.
		> 
		> I have been married to Linda for 37 years.  She has borne
the brunt of my
		> depression for all that time.  We have three children, all
of whom are
		well
		> educated (Jason a city planner, Stevyn a pharmacist, and
David a Ph.D.
		> student in organo-metallic chemistry at McGill
unioversity).  None of them
		> have inherited my depression.  All of them love and
respect me and will
		miss
		> me when I die.
		> 
		> Mentall illness is no more a single entity than any other
branch of human
		> activity.  Both smallpox and the common cold are viral
diseases, but why
		> would anyone in their right mind think that a person with
a cold is as
		> dangerous as a person with smallpox.  Please get a
perspective.  Mental
		> illness ranges from the inocuous to the dangerous.  Most
people alive
		(that
		> means you) have some kind of a neurosis, and all neuroses
are a form of
		> mental illness.  From very personal experience, I can tell
you that those
		> who suffer most from mentall illnesses are the people with
it and those
		who
		> love them.  An example is the poor progress of the
StainsFile web site, on
		> which I have been unable to work for a year.
		> 
		> I am most certainly not ashamed of my mental state.  In
fact, I am quite
		> proud of the fact that I have been strong willed enough to
rise above my
		> handicap.  I think I have made a contribution to society.
I have never
		hit
		> anyone.  I have never killed anyone.  I treat others with
respect.  Why
		> should I be discriminated against because someone else has
a hangup (a
		> neurosis) about emotional disorders?  Why should I be
fired when it is
		your
		> problem?  Would you fire me if I had one arm?  Would you
refuse to work
		with
		> me if I stuttered?  Would you send me to the back of the
bus if I were
		> black?  Please tell me how discriminating against me
because of my
		> depression is any different from those things.
		> 
		> Remember, we are all innocent until proven guilty.  Please
do not fire me
		> until I have done something worth being fired for.  As for
not hiring
		> someone because in a ten minute interview, an interviewer
with no
		> psychiatric training decides the candidate is nuts,
please, get a life!
		> 
		> I would request that people think before they post
comments that are
		> demeaning to those like me.
		> 
		> Bryan Llewellyn
		> Depressive, and absolutely NOT ashamed of it.

		- -- 
		Thomas J. Kuwahara - Senior Immunohistochemist
		Resolution Sciences Corporation - http:www.resolve3d.com
		3801 Sacramento St., Suite 621, San Francisco, CA  94118
		T: 415/750-2307 F: 415/750-2332  E: tom@resolve3d.com


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 10:33:49 -0500
		From: GSennello@nexstar.com
		Subject: Re: Slightly OT: Live cells anyone?


		You probably need to speak to someone about cell culture.  I
have collected
		induced tumors from mice (using sterile technique) with a
scalpel and then
		placed them in a small amount of  the appropriate tissue
culture media and
		minced them into a slurry which was then put in a flask to
grow.  Many cell
		lines do very well.  From your message I can't see where the
100 micron
		slices  comes into play ?  Why? And yes I think most
embedding media would
		kill cells although most cell lines can be frozen if done
properly.


	

		            
		                    Jerry

		            
		                    Chi-Yuan Hu          To:
histonet@pathology.swmed.edu 
		            
		                    <ragnarok@ruf        cc:

		            
		                    .rice.edu>           Subject:
Slightly OT: Live cells
		anyone?      
	

		            
		                    07/11/00

		            
		                    06:53 AM

		            
	

		            
	

		            




		Hello Everyone,

		I am looking for a way to isolate cells from thin slices (at
100 um
		thick) and to start a cell line from these cells.  Everyone
I've spoken to
		says that the fresh tissue has to be embedded for slicing in
a microtome
		cryostat, though slices of 100 um might crack...  I also do
not know if
		the embedding medium is going to kill my cells.

		If anyone knows how to use the microtome (or cryotome) to
get thick slices
		of live cells please let me know.  Your help is greatly
appreciated!

		Jerry Hu








	
----------------------------------------------------------------------

		Date: 11 Jul 2000 10:34:12 -0500
		From: "Weems, Joyce" <JWEEMS@sjha.org>
		Subject: RE: consult charges, documentation

		We have a form that the drs. office faxes to us - with
physician signature,
		that we use for this sort of thing.
		Joyce Weems
		Pathology Manager
		Saint Joseph's Hospital of Atlanta


			-----Original Message-----
			From:	hkcormier [SMTP:hkcorm@banet.net]
			Sent:	Monday, July 10, 2000 7:14 PM
			To:	histonet@pathology.swmed.edu
			Subject:	consult charges, documentation

			Hello All,
			I have an interesting question for you all, our
corporate compliance
			program is currently looking at path consult
charges...how does
		everyone
			(anyone) document physician requests for consults.
By this I mean
		the
			patient's doctor calls the office and requests that
the case be sent
		out
			to another pathologist, not the "house" pathologist
sending our for
		a
			second opinion. Our issue is that we are a fairly
small hospital
		with
			only 2 pathologist, our pathologists will routinely
send out cases
		for a
			second opinion, on say bone marrows to the regions
top bone marrow
		guy.
			The local visiting oncologist and ocasionally a
local surgeon -
		primary
			care physician will request that a case be sent out,
some where
		else.
			The issue is, is that it is usually a phone call
from the doctor or
			doctor's staff and we never receive any written
order or request.
		This
			our course would slow us all down and wreck havoc
with our customer
			service policy. Our corporate people feel that
without documentation
			from somewhere, this may be construed as fraud.  Any
ideas? Any one
		ever
			deal with this before? Any suggestions? Thanks!
Kathy
			


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:22:02 -0500
		From: Diane.Burica@advocatehealth.com (Diane Burica)
		Subject: unssubscribe



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:54:23 -0500
		From: Sheila Poellein <sheila_poellein@deaconess.com>
		Subject: AP computer systems

		We are a current Sunquest computer user looking for a new
system since they
		will cease support of their AP product in the near future.
Our three top
		choices are Copath, Tamtron, and SCC's Softpath.  I would
appreciate any and
		all comments of these three systems especially of the SCC
system of which we
		are least familiar.   If you are a user of any of these,
what do you
		like/dislike about your system.   Thanks in advance.
Sheila



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:54:39 -0500
		From: "Bonnie P Whitaker" <Bonnie.P.Whitaker@uth.tmc.edu>
		Subject: help with Syndecan-1,  FA1, SDF1, NRG-1

		Hi,

		One of the pathologists that I am working with wants to do a
project
		involving the following antibodies:

		Syndecan-1
		FA1 (fetal antigen1)
		SDF1 (stromal cell-derived factor 1)
		NRG-1 (neuregulins)

		Doe anyone have any experience with these?  If so, please
recommend sources
		and share any tips for getting these to work.

		Thanks,
		Bonnie Whitaker
		UT--Houston



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:54:54 -0500
		From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
		Subject: Re: IH on Liver Tissue

		There is a peroxidase quench that will block ALL forms of
peroxidase,
		totally.  This is the glucose oxidase method.   

		Hsu H-M, et al Am J Pathology 188:209-217,1984

		Andrew SM and Jasani G Histchem J 19:426-430, 1987

		Vector provided me with the exact protocol


		>Date: Mon, 10 Jul 2000 14:43:03 -0400
		>From: Jeff Crews <jcrews@organo.com>
		>Subject: Re: IH on Liver Tissue
		>To: kkdulany@unmc.edu, histonet@pathology.swmed.edu
		>
		>     With extremely bloody tissue like spleen or liver, it
is sometimes 
		>     impossible to quench all of the peroxidase present in
the blood. Try 
		>     an alkaline-phoshatase detection system.
		>     
		>     Jeffrey Crews, HTL (ASCP)
		>     Organogenesis, Inc.
		>
		>
		>______________________________ Reply Separator
		_________________________________
		>Subject: IH on Liver Tissue 
		>Author:  <kkdulany@unmc.edu> at internet
		>Date:    07/10/2000 11:03 AM
		>
		>
		>How can you successfully perform immunohistochemistry on
slides of liver 
		>tissue using horse-radish peroxidase and DAB?  How do you
quench the 
		>endogenous peroxidase in the tissue so everything doesn't
turn brown?  I've 
		>tried 3, 10 and 15 % H2O2 in both methanol and di water for
20 min. up to 1 
		>hour with no success.  The tissues are formalin fixed and
embeded in 
		>paraffin.  We then used a pH 6.0 citrate buffer/microwave
antigen retrieval 
		>system for our primary Ab detection.  Even with all that
the endogenous 
		>enzyme caused the false positive with the DAB.  Anyone have
any 
		>suggestions?
		>Thank you for all replies.
		>Karen in Omaha
		>     
		>     
		>     
		>
		>
		>
		>
		Gayle Callis
		Veterinary Molecular Biology
		Montana State University
		Bozeman MT 59717-3610
		406 994-4705
		406 994-4303


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:55:27 -0500
		From: Cynthia Favara <cfavara@niaid.nih.gov>
		Subject: RE: Slightly OT: Live cells anyone?

		Jerry,
			You do not have to embedd to cut frozen sections. I
am presuming
		[always dangerous] you are working with a piece of tissue.
Tissue can be
		flash frozen in liguid nitrogen and can then be put on a
chuck for cutting.
		It is possible to do this so the tissue remains frozen by
dipping the chuck
		in liquid nitrogen and slowly building a little throne for
the tissue. Make
		sure the tissue remains frozen as well. 100um pieces may
crack but since you
		are going to isolate cells I would not think cracking would
matter.
		Good Luck,
		Cynthia Favara 
		NIAID/RML/LPVD 
		903 South 4th Street 
		Hamilton, MT 59840 
		PH: 406-363-9317 
		FAX: 406-363-9286 

		e-mail: CF98d@nih.gov <mailto:CF98d@nih.gov> 

		 



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:55:41 -0500
		From: Cynthia Favara <cfavara@niaid.nih.gov>
		Subject: RE: whole eye serial sections

		I took a workshop from a group in Tennessee that had a
wonderful
		presentation on eyes. I could probably find the name if you
desire.
		Cynthia Favara 
		NIAID/RML/LPVD 
		903 South 4th Street 
		Hamilton, MT 59840 
		PH: 406-363-9317 
		FAX: 406-363-9286 

		e-mail: CF98d@nih.gov <mailto:CF98d@nih.gov> 

		 



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:55:59 -0500
		From: Cynthia Favara <cfavara@niaid.nih.gov>
		Subject: RE: IH on Liver Tissue

		I do not know what species you are working with but I do IHC
with HRP on
		murine liver all the time with no difficulty at all.
Sections are cut at 4um
		and endogenous peroxidase is blocked for 15 minutes in 0.3%
vol/vol hydrogen
		peroxide in PBS-0.02% Tween for 15 minutes.
		Cynthia Favara 
		NIAID/RML/LPVD 
		903 South 4th Street 
		Hamilton, MT 59840 
		PH: 406-363-9317 
		FAX: 406-363-9286 

		e-mail: CF98d@nih.gov <mailto:CF98d@nih.gov> 

		 



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:56:15 -0500
		From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
		Subject: Sheep antibodies

		SeroTec at www.serotec.co.uk


		Gayle Callis
		Veterinary Molecular Biology
		Montana State University
		Bozeman MT 59717-3610
		406 994-4705
		406 994-4303


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:56:39 -0500
		From: Nick_Madary@hgsi.com
		Subject: Open until filled Histology position

		Histology Position Available
		Human Genome Sciences, Inc
		Salary Position M-F some weekends

		Must be proficient in the following areas:
		Performing small animal necropsies and gross dissection,
		All aspects of research veterinary histology(including
cryotomy)
		Animal and Human Immunohistochemistry

		Certification required
		ISH experience and degree desirable

		Send Resume to Human Genome Sciences, Inc
		Human Resources Attn: Neubela Dahi
		9410 Key West Ave
		Rockville, Md 20850

		In your cover letter mention this ad and reference Nick
Madary

		For question regarding this position you may contact Nick
Madary via Email
		at Nick_Madary@HGSI.COM or call 301 610 5790 ext 3537



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:57:05 -0500
		From: "Gamble,Marilyn S" <Marilyn.S.Gamble@kp.org>
		Subject: RE: NSH Scholarships and Starting a Histology
program

		NSH Scholarships, for continuing education, are made
available through NSH,
		and the gererous sponsorship of many of our vendor
companies.  The criteria,
		and deadline date, is established by the NSH Awards
Committee, for
		nominees/applicants.  The information is published annually
in "NSH In
		Action" (sent to all NSH members).   It is too late to apply
for this year's
		awards, but I would encourage members to become more
involved in this
		process.  The percentage of members who apply and/or
nominate someone is
		very low.  

			-----Original Message-----
			From:	DENISE BLAND-PIONTEK
[SMTP:DBLANDP1@FAIRVIEW.ORG]
			Sent:	Monday, July 10, 2000 5:01 PM
			To:	histonet@pathology.swmed.edu
			Subject:	NSH Scholarships and Starting a
Histology program

			I'm asking these questions for a fellow
histotechnologist, Pam
		Buzzard, who resides in Georgia. She will be joining the
Histonet soon. Does
		the NSH offer any scholarships for attending nationals? Does
anyone have a
		suggested curriculum with what each course should entail as
far as starting
		a histotech program is concerned?  She has been asked by a
local college to
		initiate a program, but has little info to go on. I have
already suggested
		attending the workshop that is going to be offered at
Nationals this year. I
		will inform her of all answers posted on the histonet, but
she can otherwise
		be contacted at PDBUZZ68@aol.com. 
			Thank you, 
			Denise Bland-Piontek HTL (ASCP)
			Fairview University Medical Center
			Minneapolis, MN

			


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:57:33 -0500
		From: "Pam M. Dilbeck" <pmd@vetmed.wsu.edu>
		Subject: Re: Marker for T-cells in sheep

		Jenny--I have run Dako's CD3 A452 on several different
species (canine,
		feline, equine, etc) but never tried on ovines.  You might
try it--run a
		lymph node as a control tissue.  I use citrate buffer with
steam for
		antigen retrieval and incuabate overnight in refrig. (use at
1:50,000).  I
		use Signet's ultra streptavidin (USA) detection and AEC for
the
		chromogen.  Let me know if you would like more information.
		Pam



		On Tue, 11 Jul 2000, Jenny Molde wrote:

		> Dear Histonetters
		> I am looking for a lymphocyte marker for sheep. Anyone out
there had any
		> experience with this. Many thanks.
		> 
		> Jenny Molde
		> 
		> 
		> 
		> 

			Pam Dilbeck
			Immunohistochemistry Section
			Washington Animal Disease Diagnostic Laboratory



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:57:51 -0500
		From: "Ronnie Houston" <wee_rory@hotmail.com>
		Subject: non-histology topics

		Please get back to what Histonet was set up for - an
exchange of matters 
		related to Histotechnology.

		The recent discussions, lamblastings and character
assassinations have no 
		place on Histonet.
		Sure, people have rights to express their feelings, but
Histonet is not the 
		correct avenue for this.

		I highly commend Brian's stance and courage, and if people
had the common 
		sense to limit Histonet topics to Histology, he would not
have  been forced 
		to make the painful decision to put forward publicly his

		Let's keep the list histo-related from now on to avoid this
unpleasant and 
		unnecessary situation arising again.


		Ronnie Houston
		Cytochemistry & Molecular Pathology
		Texas Scottish Rite Hospital for Children
		2222 Welborn Street
		Dallas, TX 75219
		(214) 559 7744
		(214) 559 7768 - fax
		rhouston@tsrh.org

	
________________________________________________________________________
		Get Your Private, Free E-mail from MSN Hotmail at
http://www.hotmail.com



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:58:07 -0500
		From: "Gamble,Marilyn S" <Marilyn.S.Gamble@kp.org>
		Subject: More Than A Gut Feeling

		As part of the management workshop that I taught for several
years, I, also,
		utilized the "More Than a Gut Feeling" information. Once you
have
		established your hiring criteria,  It teaches you how to
develop questions,
		interview, and to obtain reponses that will give you insight
into past
		behaviors (attitudes, interpersonal skills, dealing with
problems, etc.) and
		performance.  Past behavior/performance responses can
predict future
		behaviors/performance. 

		It is not a "screen" for mental illness,  nor would
eliminate a good
		employee from being hired into the workplace.

			-----Original Message-----
			From:	Janice Mahoney [SMTP:JMAHONEY@alegent.org]
			Sent:	Monday, July 10, 2000 11:15 AM
			To:	bryand@netbistro.com;
histonet@pathology.swmed.edu;
		tom@resolve3d.com
			Subject:	Re: Mental illness: the other side
-Reply


			I don't believe any part of Vinnie's workshop goes
into determining
		if a person has a mental illness.  What it does go into is
whether a job
		candidate has the correct personaloity traits to work in the
sometimes
		highly stresssful field of Histology.  Not every person
posssesses the
		ability to "fit in" in our labs.  We usually have to have
the ability to
		work as a team, communicate effectively, make critical
decisions, work under
		stress, be self motivated etc., etc.  
			As far a having the potential for violence, there
are some
		indicators and situations that may make the probability
increase.
		Discrimination is not the issue.  Supervisors need to be
aware of an
		employees emotional state as well as their work.  Its no
different than
		being aware of an employee who is having personal poroblems
that  cause them
		to make errors in thier technical work.  A supervisor should
be just as
		aware of an employees mental state that may cause him/her to
injur or become
		injured.  
			Jan Mahoney


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:58:22 -0500
		From: Medilex123@aol.com
		Subject: Re: Foetuses

		Carmen,
		I know we have to be concerned about the feling of grieving
parents but I 
		would feel a little concerned about dressing up a fixed
fetus, on the grounds 
		of  health and safety as well as the aesthetic look of a
fetus after 
		fixation. 

		When I worked in a maternity hospital in the UK, parents who
lost babies and 
		fetuses didn't have to ask whether they could have a photo
or not, a polaroid 
		was routinely taken by the nursing/midwifery staff along
with a foot-print 
		which was placed onto a small piece of card with the babies
name on it. They 
		were offered the photo and print if they wanted it. 



		Regards,


		Glyn Woodward


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:58:48 -0500
		From: Dana Settembre <settembr@UMDNJ.EDU>
		Subject: Re: Immunos on Cytology Specimens

		On Thu, 6 Jul 2000 DRitter@stmarygj.com wrote:

		> I would appreciate any feed back on Immunohisto procedures
done on
		> cytology smears,.cytospins etc.
		> We recently purchased a Biogenex Optimax Immuno stainer
(and love it).
		> I heard that you could fix smears etc. in cold acetone and
then start
		> your normal run procedure. Has any one used anything else
and had great
		> results?
		> Thanks
		> Deb Ritter, HT ASCP
		> St. Marys Hosp.
		> Grand Junction, Colorado
		> 

		Hello Deb,
		When I run cytology smears I start them at buffer and join
them with my
		regular paraffins at the H2O2 step.

		When I get them, usually I get them in water, I put them in
buffer.  In
		the mean time my paraffins are deparaffinzing to water and
getting
		pretreated as necessary and go into buffer and H2O2 with the
smears.

		Dana Settembre
		Immunohistochemistry Lab
		Pathology Department
		University Hospital
		Newark,  New Jersey
		USA



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:59:07 -0500
		From: Dana Settembre <settembr@UMDNJ.EDU>
		Subject: Re: IH on Liver Tissue

		On Mon, 10 Jul 2000 kkdulany@unmc.edu wrote:

		> How can you successfully perform immunohistochemistry on
slides of liver
		> tissue using horse-radish peroxidase and DAB?  How do you
quench the
		> endogenous peroxidase in the tissue so everything doesn't
turn brown?  I've
		> tried 3, 10 and 15 % H2O2 in both methanol and di water
for 20 min. up to 1
		> hour with no success.  The tissues are formalin fixed and
embeded in
		> paraffin.  We then used a pH 6.0 citrate buffer/microwave
antigen retrieval
		> system for our primary Ab detection.  Even with all that
the endogenous
		> enzyme caused the false positive with the DAB.  Anyone
have any
		> suggestions?
		> Thank you for all replies.
		> Karen in Omaha
		> 
		> 

		Karen,
		What antibody are you using?  Everythin you do sounds like
what I do but
		maybe you can skip the antigen retrieval step and see what
happens.  My
		guess is that omitting that will help.

		Dana Settembre
		Immunohistochemistry Lab
		Pathology Department
		University Hospital
		Newark,  New Jersey
		USA



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 15:59:39 -0500
		From: "Jennings-Siena, Debbie"
<ds.jennings-siena@baylordallas.edu>
		Subject: RE: consult charges, documentation

		Just wanted to let everyone know that on June 21,2000 the
American Society
		of Clinical Pathologists hosted a consensus conference on
second opinions in
		diagnostic anatomic pathology.  Ten panelist from around the
country,
		including eight pathologists of various disciplines, a
patient represenative
		and a surgeon met in Washington D.C. to debate the who,
what, how and when
		of second opinions in ap.  They felt that it was important
to determine when
		second opinion review of Anatomic pathology slides is
warranted for patient
		care.  The consensus conference is part of the ASCP Patient
Safety
		Initiative, which was created in response to a report on
medical errors
		released late last year by the Institute of Medicine.  The
Panelists debated
		which patients need second opinions, which diagnoses need
more frequent
		review, and cost and payment issues.  The proceedings of the
conference are
		expected to be published in an upcoming issue of the
American Journal of
		Clinical Pathology and on the ASCP web site (www.ASCP.org).


		This was reported in the Washington Report, Dated July 5,
2000.  I hope that
		when this information is published that it will be helpful,
although not a
		total answer to your question.

		Debbie J. Siena
		NSH Legislative Chairperson
		Baylor University Medical Center
		Dallas, TX 75246

		- -----Original Message-----
		From: hkcormier [mailto:hkcorm@banet.net]
		Sent: Monday, July 10, 2000 6:14 PM
		To: histonet@pathology.swmed.edu
		Subject: consult charges, documentation


		Hello All,
		I have an interesting question for you all, our corporate
compliance
		program is currently looking at path consult charges...how
does everyone
		(anyone) document physician requests for consults. By this I
mean the
		patient's doctor calls the office and requests that the case
be sent out
		to another pathologist, not the "house" pathologist sending
our for a
		second opinion. Our issue is that we are a fairly small
hospital  with
		only 2 pathologist, our pathologists will routinely send out
cases for a
		second opinion, on say bone marrows to the regions top bone
marrow guy.
		The local visiting oncologist and ocasionally a local
surgeon - primary
		care physician will request that a case be sent out, some
where else.
		The issue is, is that it is usually a phone call from the
doctor or
		doctor's staff and we never receive any written order or
request. This
		our course would slow us all down and wreck havoc with our
customer
		service policy. Our corporate people feel that without
documentation
		from somewhere, this may be construed as fraud.  Any ideas?
Any one ever
		deal with this before? Any suggestions? Thanks!  Kathy



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 16:00:16 -0500
		From: "Colbert, Laurie" <LColbert@phsca.org>
		Subject: Cat Scratch Controls

		Does anyone know where I can purchase cat scratch controls?
Thanks in
		advance!

		Laurie Colbert
		Providence Saint Joseph Med Ctr
		Burbank, CA  91505


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 16:00:56 -0500
		From: "Goodwin, Diana" <DGoodwin@CHSNJ.org>
		Subject: RE: Alcian Blue for ... [urban myths]

			Gentlemen:

			After reading through your e-mail threads, I hurried
back to my 
			ancient storage closet where I keep solid reagents
now, since
		ready made 
			solutions became available.  Lo and behold, what do
I find but a
		dusty jar 
			of Alcian Blue 8GX!  

			Jim, please let me know when the price reaches that
of gold!


		Diana Goodwin,  HT
		Trenton,  NJ


		> ----------
		> From: 	Mark Ray[SMTP:darkdaym@earthlink.net]
		> Sent: 	Friday, July 07, 2000 2:47 PM
		> To: 	jim; J. A. Kiernan
		> Cc: 	Histonet
		> Subject: 	RE: Alcian Blue for ... [urban myths]
		> 
		> John,
		> 
		> I was not quite correct in saying that Aldrich had sold
out their last
		> batch of Alcian Blue 8GX.  At the present moment they have
1x5g and
		> 1x25g
		> cat no 236551 in stock.  When we  attempted to purchase a
		> manufacturing
		> quantity this spring, they told us that it was
unavailable.  I guess
		> they
		> still had some small packages, but it looks bleak for the
present.
		> Hopefully the pyridine derivative will become generally
available.
		> 
		> Mark Ray
		> EK Industries  
		> 
		> 
		> > [Original Message]
		> > From: J. A. Kiernan <jkiernan@julian.uwo.ca>
		> > To: jim <jim@proscitech.com.au>
		> > Cc: Histonet <Histonet@pathology.swmed.edu>
		> > Date: 7/7/00 1:30:20 AM
		> > Subject: RE: Alcian Blue for ... [urban myths]
		> >
		> > On Thu, 6 Jul 2000, Jim Darley wrote:
		> > 
		> > > Here is the note from our wholesaler/ manufacturer:
		> > > 
		> > > "Thank you for your E Mail concerning Alcian Blue 8GX.
As per
		> previous
		> > > correspondence the problem is with us and will not
disappear.
		> > > The last 3 years we found some old ICI material (low
dye content
		> 10-20%), which 
		> > > we upgraded. That has come to an end; no company in
the world to
		> our
		> knowledge 
		> > > is producing the material, textile quality or stains
quality. ..."
		> > 
		> > > ... a 1996 CD ROM will not reveal anything that has
happened
		> during 
		> > > the past three years.
		> > 
		> >   That's true; I don't have the 1999 version of the C.I.
CD-ROM.
		> >   However, on May 11th 1999 the Biol Stain Commission
certified a 
		> >   batch of alcian blue 8GX for the Aldrich Chemical
Company. See
		> >   Biotech. & Histochem. 74 (5) p. 274 (Sept 1999). So
there must
		> >   have been some new stuff around last year.
		> > 
		> >   Alcian blue 8GX (dye content approx 50%) is in the
Sigma
		> >   and Aldrich catalogues for 2000-2001; same price as
		> >   haematoxylin. (Curiously, both dyes are about $10
cheaper for
		> >   25 grams if you buy from Sigma rather than Aldrich.
Strange,
		> >   when they're the same company! In the Fluka catalogue
for
		> >   1999-2000 - also the same firm, I think - the prices
of both
		> >   dyes are some $20 higher than Aldrich's.) Aldrich also
supply
		> >   a "pyridine variant" of alcian blue, which is more
expensive,
		> >   and in the past they sold another variant called
"alcec blue,"
		> >   which could also be used for the same staining methods
(see 
		> >   F. Green 1991: The Sigma-Aldrich Handbook of Stains,
Dyes and
		> >   Indicators, p. 69-72).  
		> > 
		> >   A low dye content such as the 10-20% in your old ICI
material
		> >   is not necessarily a bad thing for alcian blue, and
batches
		> >   with high dye content (70% +) often perform badly. It
seems
		> >   likely that the additives (dextrin, boric acid etc)
are
		> >   needed in adequate amount.
		> > 
		> >  John A. Kiernan,
		> >  Department of Anatomy & Cell Biology,
		> >  The University of Western Ontario,
		> >  LONDON,  Canada  N6A 5C1
		> > 
		> > 
		> > 
		> 
		> 
		> 
		> --- Mark Ray
		> --- darkdaym@earthlink.net
		> --- EarthLink: It's your Internet.
		> 
		> 
		> 


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 16:01:44 -0500
		From: Maria Mejia <maria@mail.ski.org>
		Subject: RE: whole eye serial sections

		thank you for responding, but the lab with the rabbit whole
eyes
		found a lab to do the work in Bay Area.
		regards
		maria mejia


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 16:02:03 -0500
		From: Maria Mejia <maria@mail.ski.org>
		Subject: whole eye serial sections

		I would like to thank very much all those individuals that
responded
		to my message on serial sections of rabbit whole eyes. The
lab
		that is need of this work has found a lab to do it. Again, I
		thank everyone on the histonet.
		regards
		Maria Mejia
		Smith-Kettlewell Eye Res. Inst.
		S.F. CA


	
----------------------------------------------------------------------

		Date: 11 Jul 2000 16:04:50 -0500
		From: kkdulany@unmc.edu
		Subject: IH on Liver Tissue-- Thank you all

		I think this histonet is the best idea yet for continuing
education.  In
		response to my question about excessive background staining,
you wonderful
		people  took your time to help.  I had 14  replies in the
first hour after
		I wrote and today I had 10 more.  Thank you all so much.  I
had not
		considered biotin staining as my problem as I am rather new
at some of
		these stains but I have already order a new kit to block the
biotin.
		Previously I had  stained lots of slides  but mostly  it was
on pancreas
		tissue using insulin, glucagon,  somatastatin, and
pancreatic poly peptide
		and these did not need  any pre- treatment or digestions.
Thank you all.
		Karen in Omaha



	
----------------------------------------------------------------------

		Date: 11 Jul 2000 16:05:27 -0500
		From: DELONG_CYNTHIA_A@LILLY.COM
		Subject: Zinc Formalin vs 4% Paraformaldehyde

		Hello everyone,

		I have a fixation question regarding saline perfused brains.
What is the
		difference in drop fixing brains in zinc formalin vs 4%
Parafomaldehyde?
		Or is there?      These are then cryo protected and froze in
liquid
		nitrogen.   It seems as though zinc produces the same
results.  Are certain
		antigens harder to retrieve?

		Just curious
		Cindy




		Here are the messages received yesterday!