Are you making it up in DH2O or 100% Ethanol?

We use a 5% Alcoholic Soln.

If its too "thin", then leave the top off for a while and it will
thicken-up.

David Taylor
Drs King & Mower
Adelaide, SA
Australia

-----Original Message-----
From: ghildebrand@yorkhospital.edu [mailto:ghildebrand@yorkhospital.edu]
Sent: Tuesday, 4 July 2000 05:13
To: a i d a n s c h u r r
Cc: histonet@pathology.swmed.edu
Subject: Re: Alcian Blue for Tissue Marking


There is a system called "the Davidson Marking System" sold by Bradley
products, Inc. P.O. BOX 201405, Bloomington, MN 55420.
Shandon also has tissue marking dyes available. Their address is ; 171
Industrial Dr. Pittsburgh, PA USA phone: 412-788-1133.


On Fri, 30 Jun 2000, a i d a n s c h u r r wrote:

> Hi all,
> 
> We use a 5% solution of Alcian Blue for tissue marking (margins 
> mostly).  We now have a new pathologist who says that this 
> solution is not "thick" enough.  Since 5g/100mL is about the 
> maximum solubility for A. blue, I am at a bit of a loss.  Any 
> suggestions for this, or for alternatives would be appreciated.  ps 
> she doesn't like using indian ink either!
> 
> Cheers,
> Aidan
> 
> ___________________________________________________
> shin: device for finding furniture in the dark...
> ___________________________________________________
> a i d a n   c   s c h u r r 
>      mlso,  histology department
>       hutt valley health
>        lower hutt, new zealand
> 
>      ph.  ++64 4 5709173
>      fax  ++64 4 5709214
> ___________________________________________________
> 
> 
>