RE: Alcian Blue for ... [urban myths]
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From: | jim <jim@proscitech.com.au> |
To: | "'J. A. Kiernan'" <jkiernan@julian.uwo.ca>, Histonet <Histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Here is the note from our wholesaler/ manufacturer:
"Thank you for your E Mail concerning Alcian Blue 8GX. As per previous
correspondence the problem is with us and will not disappear.
The last 3 years we found some old ICI material (low dye content 10-20%), which
we upgraded. That has come to an end; no company in the world to our knowledge
is producing the material, textile quality or stains quality. I checked with
the Stains Commission again today, no change. Mr. John Kiernan may be a guru on
stains, however, he is not aware of commercial realities. I stand by all the
comments we have made in the past, nothing has changed."
I am only the messenger and have zero interest in manufacturing a shortage. I
should add that a 1996 CD ROM will not reveal anything that has happened during
the past three years.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service@proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com
On Wednesday, July 05, 2000 1:39 AM, J. A. Kiernan
[SMTP:jkiernan@julian.uwo.ca] wrote:
> On Sat, 1 Jul 2000, jim wrote:
>
> > I think that the concentration of Alcian Blue will soon be academic. ...
> > ... ProSciTech has no more Alcian Blue available. "
>
> This was aired on Histonet several months ago, and major
> suppliers indicated that there was no impending shortage of
> the dye. The Colour Index (CD-ROM, 1996) indicates that
> it is still manufactured by 2 or 3 companies as a textile
> dye, under various trade names. Any supplier of biological
> stains can buy the textile dye, test it as a stain, and
> sell it as alcian blue.
>
> The Biological Stain Commission regularly tests batches
> of alcian blue submitted by vendors and certifies them
> if they are OK for staining. Basic copper phthalocyanine
> dyes labelled alcian blue have always been pretty variable,
> so it's best to buy from a certified batch.
>
> There's nothing new in perceived shortages of biological
> stains. In the early 1970s haematoxylin was supposedly in
> short supply (= prices up) for such mythical reasons as a
> disease of Carribean logwood trees and a sunk ship that
> carried the world's harvest of Haematoxylon heartwood.
> In the early 1990s there was a similar non-shortage of
> light green SF (supposedly because of a banned toxic
> compound used in its manufacture). The Biological Stain
> Commission soon sorted that one out (Penney & Powers 1995
> Biotechn Histochem 70:217). There was never a real
> shortage of the dye, but some bogus compounds (not certified
> by the BSC) had been sold, and they didn't work.
> (Bear in mind also that fast green FCF is better than
> light green in every way, and has identical staining
> properties.)
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON, Canada N6A 5C1
>
>
>
>
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