Lack of replies is a problem

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From:Tim Morken <>
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Greg wrote: <Hope all replies are posted for all to see!>

Personal replies are a problem on this listserver.  There have been several 
threads recently where the original questioner thanks "everyone" for their 
replies, yet I have seen few, if any, replies posted. That means we are all 
missing out on some good information.

Please, all who use this listserver, don't ever think that your replies 
don't appeal to everyone else. If it is not truely personal in nature, and 
addresses the question, we would all like to see it! I am always amazed at 
the seeminly inconsequential tidbits that appear here and turn out to be 
enormously useful later on.

Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
1600 Clifton Rd.
Atlanta, GA 30333


Phone: (404) 639-3964
FAX:  (404)639-3043

----Original Message Follows----
From: Greg Dobbin <dobbin@Upei.CA>
To: Lynn Gardner <>,
Subject: Re: Insitu Paraffin
Date: Tue, 04 Jul 2000 09:54:25 -0300 (ADT)

I Lynn,
I'm learning more and more about In situ Hyb. daily, so I am no
expert by any stretch. I hadn't thought of the waterbath as a source
of RNase contamination until you mentioned it, but is it possible that
the RNA in the tissue is protected buy the hydrophobic nature of the
paraffin?? If we can't find out for sure, I guess using DEPC treated
water wouldn't be too much of a hardship! Looking forward to
following this one up. Hope all replies are posted for all to see!
Cheers!  Greg

Date sent:      	Thu, 29 Jun 2000 18:13:12 -0500
From:           	Lynn Gardner <>
Subject:        	Insitu Paraffin
Forwarded to:

 > Dear histonetters,
 > I would be most interested in finding someone out there who does Insitu 
 > Paraffin sections routinely and who has it running consistently. I would
 > like to find out if that person or persons would be willing to share 
 > procedure with us as we would like to get it started here. We already do
 > Insitu on Frozens but would like to work with paraffin.
 > Any help is greatly appreciated. Please either e-mail the information or
 > feel free to fax it to me at 319-335-7770.
 > Thanks everyone!
 > Sincerely,
 > Lynn Gardner, HT(ASCP)

Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 Unviversity Ave.
Charlottetown, P.E.I.
Canada,  C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851

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