Keratin background in IHC

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From:Maree Gould <>
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I am supervising a masters student who is immunohistochemically staining
sheep skin - fancy finding that in New Zealand! However the keratin in the
skin is staining positive also and masking any staining we might see.  We
are staining for viral inclusions. Can anybody tell me how to block keratin
staining in the skin?  I have read this information somewhere and cannot
find it now.

Can anybody help me please?


I like deadlines, I especially like the whooshing noise they make as they
go past - James Berry

Maree Gould NZCS Dip Sci
Histology Service Unit Pathology Dept
Medical School
University Of Otago
P.O. Box 913
New Zealand

phone: 479 7152
Fax: 479 7136


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