Kangaroos & Wallaby's!?

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From:Bruce Abaloz <b.abaloz@zoology.unimelb.edu.au>
To:histoNet@Pathology.swmed.edu
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Dear Histonetters.....my name is Gratiana & I am wanting to process some VERY tiny/fragile & "precious" specimens of Tammar Wallaby Day 12 - 17 vesicles. I am hoping that someone out there has worked on specimens this fragile/minute & can kindly email me their processing schedule/procedure...one that I have a copy of from our Histologist , "layers" gradients of alcohol eg.30,50,70,90,100% in a measuring cylinder & says to put the specimen @ the top layer...BUT the 100% alcohol always goes to the top & so on until the 30% is bottom!? I have tried processing as a pellet in agar but that needs to be fine tuned & as specimens are @ this time hard to come by. I do not want to take chances. Please - if you have a suggestion I will be MOST APPRECIATIVE & THANKYOU in advance.
............Gratiana, Melbourne AUSTRALIA. 


BRUCE ABALOZ
HISTOLOGIST		      
DEPARTMENT of ZOOLOGY	           *  ph:    +61 3 93446282
The UNIVERSITY of MELBOURNE        *  fax:   +61 3 93447909 
Parkville, Victoria.3052           *  email: b.abaloz@zoology.unimelb.edu.au                                                                       
AUSTRALIA.
          OPEN THE MIND;STRENGHTHEN THE BODY;FORTIFY THE SPIRIT!!                 
                    DANCE, LIKE NO - ONE'S WATCHING!!
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