I think this histonet is the best idea yet for continuing education.  In
response to my question about excessive background staining, you wonderful
people  took your time to help.  I had 14  replies in the first hour after
I wrote and today I had 10 more.  Thank you all so much.  I had not
considered biotin staining as my problem as I am rather new at some of
these stains but I have already order a new kit to block the biotin.
Previously I had  stained lots of slides  but mostly  it was on pancreas
tissue using insulin, glucagon,  somatastatin, and pancreatic poly peptide
and these did not need  any pre- treatment or digestions. Thank you all.
Karen in Omaha