IHC on Liver

<< Previous Message | Next Message >>
From:"Coskran, Timothy M" <timothy_m_coskran@groton.pfizer.com>
To:"Histonet (E-mail)" <Histonet@pathology.swmed.edu>
Content-Type:text/plain; charset=iso-8859-1

As many of the replies indicate, the problem could be due to endogenous
biotin as well as endogenous peroxidase.  We've found that using one of the
available avidin-biotin blocking kits will help reduce the background.  You
could also change your detection from a standard ABC or LSAB to the Envision
technique which avoid the use of biotin.  We've used this kit with a few
antibodies on liver sections with good success.

Good luck,

Tim Coskran

<< Previous Message | Next Message >>