>
>
>We have processed chick hind limb for an investigator, and have 
>found that the entire section autofluoresces on all channels.  We 
>have used Eosin in our last alcohol bath for several years now, and 
>we were wondering if this could be the problem.
>
>We would also like to know, would the eosin affect 
>immunohistochemistry, and what would be the affects of eosin on 
>fluorescent staining.
>
>Is Eosin removed by deparffinization and rehydration of tissue?  Is 
>there anyway to completely remove eosin from paraffin embedded 
>tissue?
>
>How would Eosin affect other stains such as Picro-Sirius Red, 
>Alizarin Red S. , and Yause silver stain for calcium.
>