Frozen sectioning

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From:Scott McDonald <>
To:HistoNet Server <>
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Hi everyone,
            I have been asked to do some frozen sections for a research
project. These specimens need to be frozen into a block , preferably with
the least amount of organic matter involved. Would embedding them in a
saline solution pose any problems ? If this has been done before by anyone,
what concentration have you used/problems did you face? Would anyone be able
to recommend other solutions to use? The routine solution being used in the
lab I have access to , is OTC. Does anyone know the chemical makeup of this
solution (if it is available to the public)?
	Also , I have never done any cutting greater than 7um and I have been asked
to cut these at about 80um . Are there any major problems associated with
thick sectioning.
	Any help at all with this situation will be greatfully appreciated.

Rm 355
BlackBurn Bld D06
University of Sydney
N.S.W  2006
ph: [61 (02)] 93516171 or 93517569
fax: [61 (02)] 93514105

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