FW: Shanndon Cytospin Chambers

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From:"Clarke, Cheryl" <Cheryl_Clarke@sfhr.hnet.bc.ca>
To:'Histonet' <HistoNet@Pathology.swmed.edu>
Reply-To:
Content-Type:text/plain; charset=iso-8859-1


Does anyone have any information regarding purchasing "reusable" Shanndon
cytospin chambers for cytology.  We are thinking of purchasing a new
cytospin and would like the reusable chambers and and single filter cards
"double holed" if possible.  I really appreciate all your help!  Thx in
advance!


-----Original Message-----
From: HistoNet Server [mailto:histonet@pathology.swmed.edu] 
Sent: Tuesday, July 04, 2000 10:38 PM
To: HistoNet Server
Subject: Daily Digest



----------------------------------------------------------------------

Date: 4 Jul 2000 01:43:46 -0500
From: "Alex Szabo" <aszabo@gribbles.com.au>
Subject: Re: bone cement

We have found Carnoy fixative, containing alcohol, chloroform and acetic
acid, does the job very quickly. Provided you agitate the solution, the
chloroform component will dissolve the plastic-like cement in a couple of
hours.

Hope this helps


Alex Szabo
Senior Scientist Histology
Gribbles Pathology
1 Goodwood  Road
Wayville  5034
South Australia
aszabo@gribbles.com.au


- ----- Original Message -----
From: <Bobana@aol.com>
To: <histonet@pathology.swmed.edu>
Sent: Monday, July 03, 2000 1:34 PM
Subject: bone cement


> Dear histonetter,
>
> Does anyone have a technique for remowal of bone cement prior to decal and
paraffin embedding of long bones?
>
> Thanks in advance!
>
> Anna-Lena



----------------------------------------------------------------------

Date: 4 Jul 2000 01:44:15 -0500
From: Maree Gould <maree.gould@stonebow.otago.ac.nz>
Subject: Keratin background in IHC

Hi 

I am supervising a masters student who is immunohistochemically staining
sheep skin - fancy finding that in New Zealand! However the keratin in the
skin is staining positive also and masking any staining we might see.  We
are staining for viral inclusions. Can anybody tell me how to block keratin
staining in the skin?  I have read this information somewhere and cannot
find it now.

Can anybody help me please?
Thanks
Maree


_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/

I like deadlines, I especially like the whooshing noise they make as they
go past - James Berry

Maree Gould NZCS Dip Sci
Histology Service Unit Pathology Dept
Medical School
University Of Otago
P.O. Box 913
Dunedin
New Zealand

email: maree.gould@stonebow.otago.ac.nz
phone: 479 7152
Fax: 479 7136

_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/





----------------------------------------------------------------------

Date: 4 Jul 2000 01:44:31 -0500
From: Roger Moretz <stamptrain@yahoo.com>
Subject: LKB Knifemaker/1512's

Was a little too quick on the delete button a couple
of times, so I can't reply directly, and have seen
only one other reply to the queries for parts for the
LKB Glass Knifemaker and the Leica 1512's.  Leica
swallowed LKB 12 to 14 years ago, and they still make
and market a knifemaker -- an obvious descendant of
the old LKB unit.  Contact your local Leica
dealer/service group for parts.  I know that they're
on the Internet, and, since I oops'ed the originals, I
have NO idea where your local supplier might be.

Leica should be able to provide parts.  If you are in
an area where there are indenpendent service
providers, they, as well, can provide parts and get
those 1512's back to good as new.

Roger Moretz
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals

*as usual, no commercial interest, just a long time user.

__________________________________________________
Do You Yahoo!?
Kick off your party with Yahoo! Invites.
http://invites.yahoo.com/


----------------------------------------------------------------------

Date: 4 Jul 2000 05:29:01 -0500
From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
Subject: Re: Alcian blue - H & E

On Mon, 3 Jul 2000 CMD1352@aol.com wrote:

> Is anyone familiar with doing an Alcian Blue pH 2.5-H&E stain in place of
an

> H & E to see goblet cells?

  Alcian blue at pH 2.5 stains the contents of intestinal goblet
  cells a pleasing turquoise-blue. This colour is not extracted by
  later washing, staining, dehydrating etc. This is the tip of the
  iceburg of carbohydrate histochemistry with dyes. 

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1




----------------------------------------------------------------------

Date: 4 Jul 2000 07:28:13 -0500
From: "Dave Evans" <dave@rhhhistology.demon.co.uk>
Subject: Automated EM Processing; Problems with the LYNX

Dear Histonetters,

Anyone had any experience in using the Leica Lynx automated EM Processor ?

Each time we use it we always get one or two blocks under processed. 

Has anybody got experience of using other automated Em processors ?

I would be grateful for any information.

Dave Evans
Histology Dept.
Russells Hall Hospital
Dudley
West Mids




----------------------------------------------------------------------

Date: 4 Jul 2000 08:02:12 -0500
From: Greg Dobbin <dobbin@Upei.CA>
Subject: Re: Insitu Paraffin

I Lynn,
I'm learning more and more about In situ Hyb. daily, so I am no 
expert by any stretch. I hadn't thought of the waterbath as a source 
of RNase contamination until you mentioned it, but is it possible that 
the RNA in the tissue is protected buy the hydrophobic nature of the 
paraffin?? If we can't find out for sure, I guess using DEPC treated 
water wouldn't be too much of a hardship! Looking forward to 
following this one up. Hope all replies are posted for all to see! 
Cheers!  Greg

Date sent:      	Thu, 29 Jun 2000 18:13:12 -0500
From:           	Lynn Gardner <lynn-gardner@uiowa.edu>
Subject:        	Insitu Paraffin
Forwarded to:   	DOBBIN@acad1.cs.upei.ca
To:             	Histonet@pathology.swmed.edu

> Dear histonetters,
> 
> I would be most interested in finding someone out there who does Insitu on
> Paraffin sections routinely and who has it running consistently. I would
> like to find out if that person or persons would be willing to share their
> procedure with us as we would like to get it started here. We already do
> Insitu on Frozens but would like to work with paraffin. 
> 
> Any help is greatly appreciated. Please either e-mail the information or
> feel free to fax it to me at 319-335-7770.
> 
> Thanks everyone!
> 
> Sincerely,
> Lynn Gardner, HT(ASCP)
> 
> 


>
>
>
>
>
>
Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 Unviversity Ave.
Charlottetown, P.E.I.
Canada,  C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851


----------------------------------------------------------------------

Date: 4 Jul 2000 08:09:26 -0500
From: <Simon_P_Campbell@sbphrd.com>
Subject: Buffer question

Hiya all,
Has anyone heard of a Teorell and Stenhagen buffer before?
If so could you send details and/or a reference to this please?
I've looked in standard histo books and on the 'net but have come up with
anything as yet.
Thanks
Simon Campbell
Histology
SmithKline Beecham, UK




----------------------------------------------------------------------

Date: 4 Jul 2000 09:15:49 -0500
From: "Don Hammer" <donh7@earthlink.net>
Subject: Memorials

Histonetters and Other Friends,

Below is information just received.  Please pass on to those you may feel
would like this info.
We will celebrate Dr. Haggitt's life with a Memorial Service on Thursday,
July 6th.

Thanks,
Don Hammer, Retired Guy

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Donations in Dr. Haggitt's name can be made to the following:
>
> Rodger C. Haggitt, MD Memorial Research Fund
> Crohn's & Colitis Foundation of America
> 386 Park Avenue South, New York  NY, 10016-8804
>
> UW Memorial Fund (hopefully to get a chair endowed in his name)
> c/o Rodger C. Haggitt Memorial
> 1959 NE Pacific, 356100
> Seattle, WA  98195
>
> Handgun Control Inc.
> Center to Prevent Handgun Violence
> 1225 Eye Street, NW, Suite 1100
> Washington, DC  20005
>
> If I've missed anyone, please feel free to pass this along.
>
> Dawn Counts                  "Stand (for what you believe)
> Assistant to the                             before there's a permanent
> Director & Administrator             crease in your right & wrong..."
> Anatomic Pathology
> University of Washington Medical Center
> 1959 NE Pacific, BB210-C, Box 356100
> Seattle, WA  98195-6100
> Phone: (206) 598-6404   FAX: (206) 598-4928
>
>



----------------------------------------------------------------------

Date: 4 Jul 2000 10:32:02 -0500
From: Renee Seiler <horalka@iopener.net>
Subject: Re: Re:digests

Okay, how do you "replace the space"?
- ----- Original Message -----

From: histonet@pathology.swmed.edu
To: Renee Seiler <horalka@iopener.net>
Subject: Re:digests
Date: Tue, 04 Jul 2000 00:34:41 -0500 (CDT)

Thank you for using this ListSTAR listserver. 
 
 
Below is a list of the files that are available for retrieval from this 
listserver. To request any of the files, replace the space between the 
brackets with an 'x' (as in "[x]"). You may request as many files as you 
wish by marking multiple items. Each file will be returned to you as the 
enclosure of a separate mail message. 
 
- ---------------------- File List Follows ---------------------- 
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    Size is 2.6211E+4 bytes 
 
 




----------------------------------------------------------------------

Date: 4 Jul 2000 10:32:15 -0500
From: "Teri Johnson" <terij@prlnet.com>
Subject: Re: ? about HALT

We use it as well, and what I've found is that it is possible to get too
much in the waterbath.  The detergent decreases the surface tension of the
water and if there is too much your paraffin sections will sink under the
surface if you manipulate them.  We have the same size waterbath and I
usually use about 1/3 a capful.  This works well for us.  Your waterbath
temperature should depend on the melting point of your paraffin;  I like to
have my waterbath set at 45 degrees C.  I still allow the sections to
"relax" for a minute or so before mounting them on a slide.

I suspect that cornea is one of those tissues that just don't know how to
"relax".  :)

Teri Johnson, HT(ASCP)
Anatomic Pathology Supervisor
Physicians Reference Laboratory

- ----- Original Message -----
From: <Snobird75@aol.com>
To: <histonet@pathology.swmed.edu>
Sent: Monday, July 03, 2000 1:00 PM
Subject: ? about HALT


> Dear Histonet friends
> I received my free sample of HALT and have so far used it twice in my
waterbath , while cutting eye cornea's and mouse skins. I still see wrinkles
, I don't see any difference with use or without. My waterbath is set at
42-44 degress and I am using postive slides. For those of histoland that
said they like this product, is my waterbath the right temp for this to
work? Or do I need to do something else. I am using 2 capfuls in a waterbath
size 8X8.
> Thank you
> Sandi Miller HT
> MRICD Research
> Md
>



----------------------------------------------------------------------

Date: 4 Jul 2000 11:35:33 -0500
From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
Subject: RE: Alcian Blue for ... [urban myths]

On Sat, 1 Jul 2000, jim wrote:

> I think that the concentration of Alcian Blue will soon be academic. ...
> ...     ProSciTech has no more Alcian Blue available. "

  This was aired on Histonet several months ago, and major
  suppliers indicated that there was no impending shortage of
  the dye. The Colour Index (CD-ROM, 1996) indicates that
  it is still manufactured by 2 or 3 companies as a textile
  dye, under various trade names. Any supplier of biological 
  stains can buy the textile dye, test it as a stain, and 
  sell it as alcian blue. 

  The Biological Stain Commission regularly tests batches
  of alcian blue submitted by vendors and certifies them
  if they are OK for staining. Basic copper phthalocyanine
  dyes labelled alcian blue have always been pretty variable,
  so it's best to buy from a certified batch. 

  There's nothing new in perceived shortages of biological
  stains. In the early 1970s haematoxylin was supposedly in
  short supply (= prices up) for such mythical reasons as a
  disease of Carribean logwood trees and a sunk ship that
  carried the world's harvest of Haematoxylon heartwood.
  In the early 1990s there was a similar non-shortage of
  light green SF (supposedly because of a banned toxic
  compound used in its manufacture). The Biological Stain
  Commission soon sorted that one out (Penney & Powers 1995
  Biotechn Histochem 70:217). There was never a real
  shortage of the dye, but some bogus compounds (not certified
  by the BSC) had been sold, and they didn't work.
  (Bear in mind also that fast green FCF is better than 
  light green in every way, and has identical staining
  properties.)

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1






----------------------------------------------------------------------

Date: 4 Jul 2000 12:02:11 -0500
From: DDittus787@aol.com
Subject: Re: Image analysis for CerbB-2

Diana
Chromavision has a very nice package that quanitates cerb-2 and if not 
mistaken does ER/PR as well. They are out of Calif. of course.
                                                   Dana


----------------------------------------------------------------------

Date: 4 Jul 2000 12:02:25 -0500
From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
Subject: RE: Re - formaldehyde substitutes [Prefer]

On Wed, 28 Jun 2000, Petrilli, Michael wrote:

> We had problem with prefer-fixed tissue and immunos ... have
> discontinued use.

  Can you tell us all what the problem was? 

  Although the exact composition of Anatech's "Prefer" fixative
  is a trade secret, its active ingredient (glyoxal) and mode
  of action (aldehyde reactivity similar to formaldehyde's) are
  clearly explained in Anatech's literature (www.anatech.com)
  and in some peer-reviewed publications (e.g. Dapson, 1993
  Biotech Histochem 68:75-82). Glyoxal fixation has also been 
  explained in various HistoNet messages that should be findable 
  in the Archives.  

  Any problem encountered with a glyoxal-based fixative but not
  with formaldehyde would be of general interest. So, for that
  matter, would any applications for which glyoxal turns out
  to give better fixation or antigen preservation.

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1



----------------------------------------------------------------------

Date: 4 Jul 2000 12:03:00 -0500
From: Renee Seiler <horalka@iopener.net>
Subject: Re: Memorials

First of all, my sympathies to all involved; secondly, I believe your
emotions
may be clouding your good judgement by posting the anti-handgun
info.(especially on this, our day to celebrate our rights and freedom). Once
again, this is a forum for histology related queries. A simple message as to
who to contact for donations should have sufficed.
- ----- Original Message -----

From: donh7@earthlink.net
To: <Undisclosed-Recipient:@earthlink.net;>
Subject: Memorials
Date: Tue, 04 Jul 2000 07:03:55 -0700

Histonetters and Other Friends, 
 
Below is information just received.  Please pass on to those you may feel 
would like this info. 
We will celebrate Dr. Haggitt's life with a Memorial Service on Thursday, 
July 6th. 
 
Thanks, 
Don Hammer, Retired Guy 
 
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
> Donations in Dr. Haggitt's name can be made to the following: 
> 
> Rodger C. Haggitt, MD Memorial Research Fund 
> Crohn's & Colitis Foundation of America 
> 386 Park Avenue South, New York  NY, 10016-8804 
> 
> UW Memorial Fund (hopefully to get a chair endowed in his name) 
> c/o Rodger C. Haggitt Memorial 
> 1959 NE Pacific, 356100 
> Seattle, WA  98195 
> 
> Handgun Control Inc. 
> Center to Prevent Handgun Violence 
> 1225 Eye Street, NW, Suite 1100 
> Washington, DC  20005 
> 
> If I've missed anyone, please feel free to pass this along. 
> 
> Dawn Counts                  "Stand (for what you believe) 
> Assistant to the                             before there's a permanent 
> Director & Administrator             crease in your right & wrong..." 
> Anatomic Pathology 
> University of Washington Medical Center 
> 1959 NE Pacific, BB210-C, Box 356100 
> Seattle, WA  98195-6100 
> Phone: (206) 598-6404   FAX: (206) 598-4928 
> 
> 
 
 





----------------------------------------------------------------------

Date: 4 Jul 2000 14:30:32 -0500
From: "Sarah Christo" <schristo@cvm.tamu.edu>
Subject: More Than a Gut Feeling

Dear Netters,
   I think a more constructive discussion around the tragedy of the murder
of
Dr. Haggitt would revolve around the subject of mental illness in the
workplace.  I recently went through a period of time dealing with the
"co-worker from Hell" syndrome that actually we believe involved some form
of
mental illness.
   I was fortunate enough to attend Vincent Speranza's workshop in
Providence
which dealt with how to avoid hiring the wrong person.  I highly recommend
that workshop for anyone who is in the position of hiring people.  The
workshop discussed interviewing questions and follow up questions that are
used to bring out the true person during the interview process.
  I find it quite confusing in the health profession (and the country in
general) that mental illness is not taken as seriously as a physical
illness. 
Someone could be physically handicapped and still function on the job where
someone mentally handicapped may look very professional but be very
dysfunctional on the job.  And mental problems effect co-workers and your
work
team to a much greater extent.
  If the turnout at Vinnie's workshop was any indication (it was packed), I
think far too many of us have had these sad experiences on the job.   
  In sympathy,  Sarah

Sarah Christo, HT (ASCP)
Research Associate, Histology Lab
Texas A&M University
College of Veterinary Medicine
Dept. of Vet Anatomy & Public Health
College Station, TX  77843-4458
phone: (979) 845-3177
fax:  (979) 458-3499



----------------------------------------------------------------------

Date: 4 Jul 2000 15:16:41 -0500
From: JanMinshew@aol.com
Subject: Ice formations in cryostats

Hi,

Just a reminder that ice formations that grow from the chamber floor of any 
cryostat are not directly caused by a frost buildup. They are most commonly 
caused by a leak or blockage somewhere in the defrost system.  If you check 
above the ice formation you can usually detect the cause of the problem.  

You will notice these types of problems more during times of heavy use or 
high humidity because there is more frost on the fins, creating more water
to 
remove when the defrost system is activated.  If the water cannot be
directed 
correctly through the defrost pan and out the tubing it will find another
way 
to travel--usually creating a stalagmite type of growth on the chamber
floor. 
 

Jan Minshew
Technical Director 
TBS, Inc.


----------------------------------------------------------------------

Date: 4 Jul 2000 15:57:16 -0500
From: TERRYM@stjoe.on.ca
Subject: Re:Ice formations in cryostats

Please note that I shall be on vacation until Mon. July 24th. 2000.
Please contact the Main Pathology Lab at ext. 6260 for assistance in my
absence. Thank You.


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Date: 4 Jul 2000 16:41:14 -0500
From: Moira Kerr <moira.kerr@usask.ca>
Subject: 

unsubscribe




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Date: 4 Jul 2000 17:11:08 -0500
From: "Clarke Ian" <clarke.ian@virgin.net>
Subject: Re: Pathology Assistants

 I would also like information regarding remuneration of Pathology
Assistants.

Ian Clarke
Histopathology/Cytopathology Department
Craigavon Area Hospital NHS Trust.
66 Lurgan Road
BT66 5QQ
Co.Armagh
Northern Ireland
- -----Original Message-----
From: Brennan, Liam <Liam.Brennan@bll.n-i.nhs.uk>
To: HISTONET <histonet@pathology.swmed.edu>
Date: 03 July 2000 16:57
Subject: Pathology Assistants


>
>
>
>
>
>> Dear Histonetters
>>
>> I would be grateful if you could supply me with some
>> information about Pathology Assistants working in pathology
>> laboratories. The type of information I am interested in is:
>> Qualifications required eg. University degree etc. Details of
>> training, both formal and on the job training. Details of job content,
>> especially with regard to dissection of pathology specimens
>> (Range and complexity of specimens dissected?), involvment in autopsy?
>> and other duties. I am also interested in a rough guide to what
>> sort of renumeration package a Pathology Assistant could expect to be
>> offered?
> The reason being that in our Laboratory all the specimen
>dissection is done by Biomedical Scientists (what you call
>histotechnologists, I think?). We are in the process of seeking payment
>for these duties and we do not want to sell ourselves short off the
>going rate for such duties, which I understand are carried out by
>Pathology Assistants in US labs. I would also be interested in
>hearing from anyone outside the US who is doing similar work.
>
> Many thanks for your anticipated assistance.
>
>> Liam Brennan
>> Histopathology Department
>> Belfast City Hospital
>> Lisburn Road
>> Belfast, BT9 7AD
>> Northern Ireland
>>
>



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Date: 4 Jul 2000 17:11:31 -0500
From: Kelvin Yen <kelvin@kyen.org>
Subject: Methyl/Ethyl green - pyronin + Immunostaining problem

Hi,

I've been trying to immunostain for estrogen receptors using an ABC
system and then following that up with a ethyl green-pyronin(EGP)
stain and have been having problems when I combine the EGP w/ the
immunostaining.  I was wondering if anyone has a protocol that I can
use that works for them or if anyone has an experience with combining
EGP and immunostaining.  My EGP staining protocol works just right by
itself and so does the immunostaining but the combination of the two
seems to cause the EGP stain to differentiate poorly and both dyes
stain the nucleus.
Any insights would be extremely appreciated.

- -Kelvin Yen
Diamond Lab
University of California at Berkeley


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Date: 4 Jul 2000 19:29:05 -0500
From: jim <jim@proscitech.com.au>
Subject: RE: Automated EM Processing; Problems with the LYNX

EMS is now the worldwide distributor for Lynx and we are the Australian /New

Zealand agent for that instrument. Leica is no longer likely to provide
advise. 
I have not enough experience with the Lynx to solve your problem, but note
that 
the Lynx has been around for some years and has an excellent reputation, it 
works well.
Its possible that yours has a fault or that your methodology needs changing.
If 
required contact Stacie at EMS 		SGKCCK@aol.com
The listserver@MSA.microscopy.com
has more EM people hanging off it and would be a better forum for EM related

questions.
Cheers
Jim Darley
ProSciTech                 Microscopy PLUS
PO Box 111, Thuringowa  QLD  4817  Australia
Ph +61 7 4774 0370  Fax:+61 7 4789 2313  service@proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560                      www.proscitech.com

On Tuesday, July 04, 2000 10:21 PM, Dave Evans 
[SMTP:dave@rhhhistology.demon.co.uk] wrote:
> Dear Histonetters,
>
> Anyone had any experience in using the Leica Lynx automated EM Processor ?
>
> Each time we use it we always get one or two blocks under processed.
>
> Has anybody got experience of using other automated Em processors ?
>
> I would be grateful for any information.
>
> Dave Evans
> Histology Dept.
> Russells Hall Hospital
> Dudley
> West Mids
>
>



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Date: 5 Jul 2000 00:01:41 -0500
From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
Subject: Re: Methyl/Ethyl green - pyronin + Immunostaining problem

On Tue, 4 Jul 2000, Kelvin Yen wrote:

> I've been trying to immunostain for estrogen receptors using an ABC
> system and then following that up with a ethyl green-pyronin(EGP)
> stain and have been having problems when I combine the EGP w/ the
> immunostaining. ... My EGP staining protocol works just right by
> itself and so does the immunostaining but the combination of the two
> seems to cause the EGP stain to differentiate poorly and both dyes
> stain the nucleus.

If the EGP gives correct staining of DNA & RNA on similar sections,
your problem must be due to an effecto prior immunostaining. You do
not say what kind of sections you use, or how the immunostaining is 
done. If you provide more information, probably someone will come
up with an answer very quickly.

Among the things that could be messing  up your EGP counterstain
are:

  1. An antigen retrieval procedure might extract RNA, leaving only
     DNA to be stained by ethyl green and pyronine.
  2. If the estrogen receptors are in the nuclei of cells, deposition
     of antibodies and of enzymatic reaction products might interfere
     with the competitive binding of dyes by nucleic acids.
       How are you making the immunopositive sites visible? Peroxidase
     & DAB?  An alkaline phosphatase method? Fluorescence?  

Tell it all, and someone will tell you what you should be doing. 
It's pleasing to see that the true name of ethyl green is catching
on, after 25+ years!

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1




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