AW: Decalcification of rats elbows

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From:Joachim Siegmund <>
To:Ann Thornton <>,
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Hi Ann,
I worked routinely with rats. We also processed tibia and elbows
in our standart program.
We used a simple mixture of 30ml Formic acid
                            10ml HCL
                            60ml 70% Ethanol
after 1 to 1 1/2 days you wash your elbows in 70% Ethanol or 10% Formaldehyd
to prevent tissue from swelling.
To speed up your decalcification you might chance your solution sometimes!

Hope it helps,


Joachim Siegmund /BTA
105 Lakeshore Ct.
Milledgeville, GA 31061

-----Ursprungliche Nachricht-----
Von: Ann Thornton []
Gesendet: Wednesday, July 05, 2000 4:03 PM
Betreff: Decalcification of rats elbows

Hi all,

I work in a research lab, and one of our researchers would like to get in
contact with histologists who specialize in bone work.  He is working on
rats elbows, and it is important that, when decaling and cutting, none of
the cartilage is removed from the bone.

I would appreciate advise from all of you experts out there.

Ann Thornton
Departmental Manager
Department of Pathology and Molecular Medicine
Wellington School of Medicine
New Zealand

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