AW: Decalcification of rats elbows

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From:Joachim Siegmund <j.siegmund@pmail.net>
To:Ann Thornton <pathat@wnmeds.ac.nz>, histonet@pathology.swmed.edu
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Hi Ann,
I worked routinely with rats. We also processed tibia and elbows
in our standart program.
We used a simple mixture of 30ml Formic acid
                            10ml HCL
                            60ml 70% Ethanol
after 1 to 1 1/2 days you wash your elbows in 70% Ethanol or 10% Formaldehyd
to prevent tissue from swelling.
To speed up your decalcification you might chance your solution sometimes!

Hope it helps,

Joachim

Joachim Siegmund /BTA
105 Lakeshore Ct.
Milledgeville, GA 31061
Phone:(912)451-3010
Email: joachim.siegmund@gmx.net
       jo_siegmund@web.de


-----Ursprungliche Nachricht-----
Von: Ann Thornton [mailto:pathat@wnmeds.ac.nz]
Gesendet: Wednesday, July 05, 2000 4:03 PM
An: histonet@pathology.swmed.edu
Betreff: Decalcification of rats elbows


Hi all,

I work in a research lab, and one of our researchers would like to get in
contact with histologists who specialize in bone work.  He is working on
rats elbows, and it is important that, when decaling and cutting, none of
the cartilage is removed from the bone.

I would appreciate advise from all of you experts out there.

Ann
Ann Thornton
Departmental Manager
Department of Pathology and Molecular Medicine
Wellington School of Medicine
Wellington
New Zealand





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