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From:Candace Andersson <candace.andersson@css.unc.edu>


I was wondering if anyone has any knowledge of a staining procedure which
differentially stains neurons and glial cells in cerebral cortex.  I have
20 um frozen, cryostat sections of rat prefrontal cortex that have been
perfused with 2% paraformaldehyde.  I recently came across a reference
from 1968 whichdescribes a "hematoxylin lake modification" of a Grimley,
Albrecht and Micheltich procedure which uses Grimley's malachite green
solution and basic fuchsin solution, but I would appreciate any further
I need a straightforward staining procedure.  We are staining this tissue for 
sterological analysis and are trying not to shrink the tissue by more than 

Randal Brown
Dept. of Psychiatry
University of North Carolina, Chapel Hill

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