tissue shrinkage

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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I have a publication that did this with decalcified bone, also PMMA
embedded bone and the shrinkage was 25% for both (their statistics)
Fascinating article plus an eye opener on processing tissues.
I would assume if decalcified bone shrinks, soft tissues will have the
same problem.

I also noticed this when I took my HTL exam years ago, that I had to
cut in the tissues (before processing)  larger than the final dimensions
they required due to shrinkage after processing.  Never thought to
measure the differences and calculate

Gayle Callis




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