lens histology

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From:Judy Trogadis <judy@playfair.utoronto.ca> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Dear Histoexperts:

We are planning to do immunostaining on human eye lens tissue. Is there any
special fixation protocol that would prevent the lens from becoming too
brittle and difficult to section? I have also heard that the sections can

detach from slides easily. Is any substrate optimal to prevent this?
I can already tell that the technical components of this project will be
tough to work out, however, from past experience, I also know that this
group has the answer to everything.


Judy Trogadis
Eye Research Institute and
University of Toronto
Toronto Hospital, Western Div.
399 Bathurst  St.
Toronto, Canada M5T 2S8

phone: 416-603-5088
Fax:   416-603-5126
email: judy@playfair.utoronto.ca

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