frozen section folds
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From: | "Atoska S. Gentry" <gentras@vetmed.auburn.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
hello,
does anyone have a remedy for folding/overlapping of frozen brain sections
(f.s.) as they are picked up onto glass slides.
or perhaps there is something in my routine causing this. i section at 8u
using disposable blades temp. -23C -18C.
also, is there an advantage to cryoprotecting brain sections vs fresh
sections frozen in isopentane chilled with liquid nitrogen? I have not
been cryoprotecting my sections.
thanks,
Atoska S. Gentry
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