frozen section folds

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From:"Atoska S. Gentry" <> (by way of histonet)
To:histonet <>
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does anyone have a remedy for folding/overlapping of frozen brain sections
(f.s.) as they are picked up onto  glass slides.

or perhaps there is something in my routine causing this.  i section at 8u
using disposable blades temp. -23C -18C.

also, is there an advantage to cryoprotecting brain sections vs fresh
sections frozen in isopentane chilled with liquid nitrogen?  I have not
been cryoprotecting my sections.

Atoska S. Gentry

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