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From:"Atoska S. Gentry" <gentras@vetmed.auburn.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Special thanks to both Alan Bright and Gayle Callis for your replys to my
original question.

Mr. Bright i'm not sure if we have independent specimen control.  I think
our microtome only has chamber temp. control.   We have the IEC Minotome
Microtome Cryostat digital model #2488.  And to Ms. Callis we do have an
antiroll plate and the brush.

However, additionally I am wondering if either of you or someone else
know if specimen size and/or section thickness are major contributing
factors?  I noticed on yesterday that a couple of the articles I refered
to  sectioned brain at 20um and/or 40um.  I section everything at 8um and
my samples are usually 1.5cm.x1.0cm. Is there a standard for brain frozen

Atoska S. Gentry

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