Re: neutral red stainig of nuclei

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From:Mick Rentsch <ausbio@nex.com.au> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Doubt if you could get Tris at such a low pH
-----Original Message-----
From: Neal Beeman <beemanne@york.uchsc.edu>
To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
Date: Tuesday, 12 January 1999 7:08
Subject: neutral red stainig of nuclei


>I want to stain nuclei in frozen sections with neutral red.  Does anyone
>have a handy protocol?  Also, the protocol I have looked at calls for 0.1M
>acetate buffer at pH 3.0-5.5.  Can I use Tris buffer instead?
>
>Neal
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