Re: lens histology

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I don't have a lot of experience with eyes, but I have processed them with
the routine tissues and sectioned them without any problems.  They were
fixed in 10%NBF.  We did have to use larger base molds.

The paraffin blocks were rough cut and soaked in icy water like the routine
tissues.  Did not have a problem with them being brittle.  The pathologists
and the chief of opthamology liked the results.

Rande Kline HT (ASCP)
Technical Services
EM Science

Judy Trogadis <> on 01/15/99 02:40:51 PM

To: (histonet)
Subject:  lens histology

Dear Histoexperts:

We are planning to do immunostaining on human eye lens tissue. Is there any
special fixation protocol that would prevent the lens from becoming too
brittle and difficult to section? I have also heard that the sections can
detach from slides easily. Is any substrate optimal to prevent this?
I can already tell that the technical components of this project will be
tough to work out, however, from past experience, I also know that this
group has the answer to everything.


Judy Trogadis
Eye Research Institute and
University of Toronto
Toronto Hospital, Western Div.
399 Bathurst  St.
Toronto, Canada M5T 2S8

phone: 416-603-5088
Fax:   416-603-5126

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