Re: histology and the embryo

<< Previous Message | Next Message >>
From:Sarah Christo <schristo@CVM.TAMU.EDU> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

Dear Terry,
   I have been doing day 9-13 embryos in paraffin
for several years now.  They arrive in the lab in
70% ethanol (paraformaldehyde fixed).  I use a
plastic transfer pipet to pick them up out of the
test tube and filter them thru a 2 x 2" square of
lens paper.  Fold the lens paper and place in a
cassette.  Then they are processed thru 15 minute
stations on the tissue processor.  The embedding
step is the hardest part aside from sectioning of
course.  I use a hot scapel blade to take them off
the lens paper and place in the base mold.
  During sectioning, I look at every section under
the microscope to see the embryonic disc.  Very
time consuming but they have had some nice
pubs and several grad student projects out of it.
This is on pig tissue.  Let me know if you have
any questions about paraffin processing and I'll
be glad to help.  I know there isn't much in the
liturature about this subject.
Sarah, Texas A&M

Does anyone know of any seminars/workshops coming up on
the subject of embryology, including the development of the embryo
( particularly gonads ) and also Histological procedures ( paraffin,
frozens for ICC , ISH etc. ).
I have worked mostly in the area of oncology but now the work is
moving towards Genetics and Embryology, and dealing with tiny 7-10
day embryos is quite different from adult tissues.
Alternatively, are there any experts out there willing to give some
Many thanks, Terry.

Terry Hacker,
Medical Research Council,

<< Previous Message | Next Message >>