Re: exploded tissues

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From:Mick Rentsch <> (by way of histonet)
To:histonet <>
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Dear Chris,
the problem may be twofold, in that initially your fixative may not be
Isotonic with respect to the Larva and you may have to incorporate sodium
Chloride up to 0.85% in the fixative or use Ultrafiltered Sea Water as your
solvent, I suggest you try the Sodium CHloride method though to allow for
loss of tonicity in sae water due to its dilution with the water content of
The second possibility, is too high a water bath temperature or poor
infiltration with wax with residual fat solvent (be it Limonene or Xylene
Good Luck.
Regards Mike (downunder)
-----Original Message-----
From: Hendry, Chris I <>
To: 'HistoNet Discussion Group' <>
Date: Tuesday, 12 January 1999 11:51
Subject: exploded tissues

>I am doing Paraffin-embedded sections of larval and juvenile marine fish,
>and after staining (H&E), analysis of slides reveals that in several, the
>tissues have become "exploded", i.e., interstitial spaces have become
>exaggerated.  Has anyone had experience with this before?  I haven't seen
>this before.  Your input would be greatly appreciated.
>Chris Hendry
>Department of Fisheries and Oceans
>Biological Station
>St. Andrews, NB E0G 2X0 Canada
>(506) 529-8854 Phone
>(506) 529-5862 Fax
>To steal ideas from one person is plagiarism; to steal from many is

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