Re: Sectioning thick frozens

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From:Alan Bright <Bright@dial.pipex.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Dear Cynthia,

Yes you cool the knife & anti-roll plate around -10 deg. C. cooler than the
brain sample.

Happy New Year.

Alan Bright

============================================================
-----Original Message-----
From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
To: Histonet@pathology.swmed.edu <HistoNet@Pathology.swmed.edu>; 'Alan
Bright' <Bright@dial.pipex.com>
Date: Tuesday, December 29, 1998 01:51
Subject: RE: sectioning thick frozens


>Alan,
> I have been gone and have missred this discussion. My question is do
>you maintain the cryostat at -8C to -12C and then cool the knife?
>
>Cynthia Favara
>Rocky Mountain Laboratories
>903 S 4th Street
>Hamilton, MT 59840
>ph: 406-363-9317
>FAX: 406-363-9286
>e-mail: cfavara@nih.gov
>
>
>> ----------
>> From: Alan Bright[SMTP:Bright@dial.pipex.com]
>> Sent: Tuesday, December 22, 1998 7:51 AM
>> To: Histonet@pathology.swmed.edu
>> Subject: Re: sectioning thick frozens
>>
>> I cannot understand why so many Histonetters are having problems and
>> avoiding thick frozen sections ?
>>
>> The main points to follow to achieve thick frozen sections  are:
>>
>> 1) Correct tissue temperature is most important, to cold will cause
>> excessive cracking.
>>
>> 2) Brain must be sectioned between -8 to -12deg.C. & the knife
temperature
>> should be around -10deg.C. colder than the tissue. By using this method
>> sections are cut up to 300 microns routinely.
>>
>> Merry Christmas to All,
>>
>>
>> Alan Bright
>>
>> Bright Instrument Co.Ltd.
>> St Margarets Way
>> Huntingdon
>> PE18 6EB
>> England
>>
>> Tel No; 01480 454528
>> Fax No;01480 456031
>> Email ; Bright@dial.pipex.com
>>
>>
>>
>>




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