Re: Hematoxylin and histoclear

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From:Jeff Silverman <> (by way of histonet)
To:histonet <>
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Karen and Netters'
We've used Histoclear, Americlear, Hemo De, and Clearium-all limonene
brands, for 10 years and have experienced no undue fading. However, in my
experience, if you leave slides on a tray exposed to sunlight (or
fluorescent) for longer than a week or two, slides stained with limonene
will fade rather quickly, mainly the eosin.  But, if they are stored in the
files in darkness, they fade no faster than slides cleared in xylene. Also,
limonene does not dissolve Cytoseal, Permount, or other synthetic mounting
media.  If you muck up the coverslip, you must clean up the mess with
xylene and remount.

Jeff Silverman- Southampton Hospital NY USA

> From: Karen S Pawlowski <>
> To: Histonet <>
> Subject: Hematoxylin and histoclear
> Date: Monday, January 04, 1999 11:32 AM
> Hi all,
> My boss just noticed in the 1985 edition of Cellular Pathology Technique,
> Culling, Allison and Barr eds., that food oil derivatives, such as
> histoclear, will cause "haematoxylin" staining to rapidly fade.  Is this
> true?  I have been having problems with hematoxylin fading for quite some
> time and I use histoclear in my paramount all the time. 'Tho I began (8
> years ago) by using xylene, and I think those faded just as much.
> If histoclear does cause the stain to fade, are there any other
> substitutes for xylene that would work?
> Thanks in advance for your input.
> Sincerely,
> Karen Pawlowski
> Sr Research Assoc./UT Southwestern Med Ctr.
> PhD Candidate/ UT Dallas
> Texas

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