Nicolle,

I always used kodak extachrome 400 due to it's sensitivity for green (if
that's the color you are using). You will also need to set the exposure
meter to spot-metering for dark-field photography.

Below is a mesage to me from another who uses extachrom professional
1600 with good results.



*******************************

From: Robert Santoianni <Robert_Santoianni@emory.org>  Save Address
Block Sender
               To: Microscopy@Sparc5.Microscopy.Com
               Subject: IF Photography
               Date: Wed, 16 Dec 1998 07:02:26 -0500



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----------------------------------------------------------------------re:
Fluorescence Photomicrography

Your FXA may have too many "bells and whistles" for you to plow
through to understand what's happening with your photography.  With
our rather simple set up, Nikon Optiphot with Microflex UFX II, I get
excellent pictures using Kodak Professional Ektachrome 1600, shot at
1600.  Make sure your camera can spot meter, that is to be able to read
the brightest part of your field and not average the entire field.  This
way, you meter the very strongest signal, set that value in the memory
of the timer, reposition your slide for the most pleasing shot and then
expose.
 Background should be dark to black...if you have flares or glare, there
is probably a diaphram somewhere between the lamphouse and the
microscope to stop down (but not to the point of vignetting your
picture).
Good luck!  I have an FXA instruction manual with a photography section
               if you need a copy.
               Bob Santoianni
               Emory University Hospital
               Atlanta, GA
               robert_santoianni@emory.org

*********************************************************
Tim Morken, B.S., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

FAX:  (404)639-3043



----Original Message Follows----
Date: Tue, 12 Jan 1999 10:48:10 -0500
From: "Patterson, Noelle" <PattersonN@NMRIPO.NMRI.NNMC.NAVY.MIL>
Subject: Fluorescence PhotoMicroscopy
To: Histonet <Histonet@pathology.SWMED.edu>
Cc: "Williams, R" <WilliamsR@NMRIPO.NMRI.NNMC.NAVY.MIL>

I haven't done fluorescence microscopy in years.  Now, I have an =
experiment
I did yesterday that is worth taking photos of.  Can anyone give me =
help on
how to take good fluorescence photomicrographs using an Olympus Vanox?
=
I
don't even remember what film I am supposed to use. =20

I need to do this pretty soon, the slides are in the refer, in the =
dark, but
I am not sure how long they will last.  I used Vectasheild to mount
coverslips, unfortunately, they it expired in Nov. 1995...I sealed =BE =
edges
with nail polish, the 4th was on the bottom of the slide, I will seal =
that
today...if it is not too messy.  What are the bets that the FITC fades =
by
noon today, when I am finally ready to try to take pictures.

Thanks, in advance, for all your help.

Noelle Patterson
Naval Medical Research Center
Bethesda, Md
pattersonn@NMRIPO.NMRI.NNMC.NAVY.MIL








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