Re: Alcian markers
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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Content-Type: | text/plain; charset="us-ascii" |
On Thu, 14 Jan 1999, Mick Rentsch wrote:
> Try using sat. Aq. Alcian Green, Yellow or Blue (Usually about 4-5%)
The permanence of alcian blue can be ensured by exposure to alkali
(e.g. ammonia fumes). This causes hydrolysis of the dye, removing the
cationic side chain that conferred both the positive charge and the
solubility in water. In the case of alcian blue, the residual coloured
compound is copper phthalocyanine, which is insoluble in everything
(or should I have said anything?). For alcian yellow an insoluble
azo dye should remain. Alcian green is a mixture of alcians blue and
yellow. Ref: Conn's Biological Stains, ed. RD Lillie, 9th ed. 1977.
For black writing on ground glass, you can't beat an ordinary
graphite pencil, followed by transparent nail varnish to protect
against rubbing off. On smooth glass, a diamond pencil is
permanent, but it's too troublesome for large numbers of slides,
and flying ground glass granules can end up under the coverslip.
John Kiernan
London, Canada.
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