Karen,
	I have done sucrose protection with whole mouse brains  25% sucrose
in PBS and that takes overnight.

Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov


> ----------
> From: 	Karen D. Larison[SMTP:LARISONK@UONEURO.uoregon.edu]
> Sent: 	Thursday, January 07, 1999 12:38 PM
> To: 	HistoNet@Pathology.swmed.edu
> Subject: 	that sinking feeling
>
> Hello histonetters,
>
> I have a graduate student who has done some physiology on a monkey brain.
>He
> now wants to cut cryosections of this brain (electrode tracks have been
>marked
> with DiI), and has put a portion of the brain (maybe a 3-4" cube) in 30%
> sucrose and is waiting patiently for it to sink.  He's been waiting for
>over a
> week now.  My question is:  Do all brains sink in 30% sucrose, or are the
> brains of "higher" organism too myelin-rich to sink?  Any responses will be
> appreciated.  He defends in about 3 weeks, and is a bit nervous as this work
> represents his last piece of supporting data.
>
> Thanks for all your help.
>
> Karen Larison - University of Oregon
>
>
>
>