Chris,
	When you float the section do you ever have any evidence of the
tissue exploding or seperating?  Reason to ask is that i sometimes have
this happen with murine brain and skin. I have usually been able to control
this by lowering the temp of the water bath and using a flourescent light
so there is no heat from above.
	Hope you get it worked out>
Regards,

Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov


> ----------
> From: 	Hendry, Chris I[SMTP:HendryC@mar.dfo-mpo.gc.ca]
> Sent: 	Monday, January 11, 1999 5:51 AM
> To: 	'HistoNet Discussion Group'
> Subject: 	exploded tissues
>
> I am doing Paraffin-embedded sections of larval and juvenile marine fish,
> and after staining (H&E), analysis of slides reveals that in several, the
> tissues have become "exploded", i.e., interstitial spaces have become highly
> exaggerated.  Has anyone had experience with this before?  I haven't seen
> this before.  Your input would be greatly appreciated.
>
> Chris Hendry
> Department of Fisheries and Oceans
> Biological Station
> St. Andrews, NB E0G 2X0 Canada
> (506) 529-8854 Phone
> (506) 529-5862 Fax
> e-mail: hendryc@mar.dfo-mpo.gc.ca
> URL: http://www.geocities.com/CapeCanaveral/Hall/9440
>
> To steal ideas from one person is plagiarism; to steal from many is
> research.
>
>