RE: antigen retrieval

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From:Cynthia Favara <> (by way of histonet)
To:histonet <>
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	I have dried sections on slides in a 37C oven to dry them prior to
staining as well as incubated the primary antibody of a IHC procedure at 37C
and never found it to alter the adhesion of the section to the slide
adversely. 	Hope this answers your question.

Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286

> ----------
> From: 	Hagerty, Marjorie A.[]
> Sent: 	Tuesday, January 05, 1999 12:20 PM
> To: 	'Histonet'
> Subject: 	antigen retrieval
> Hi Everyone,
> It was recommended by some list members to use a 37 degree for
> incubating slides treated with certain antibodies prior to
> immunoperoxidase staining. My question is: will slides incubated this
> way stay on during steamer antigen retrieval?
> Thanks.
> Warm Regards,
> Marg
> EMC, Rancho Mirage, CA

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