RE: antigen retrieval

<< Previous Message | Next Message >>
From:Cynthia Favara <cfavara@atlas.niaid.nih.gov> (by way of histonet)
To:histonet <histonet@magicnet.net>
Reply-To:
Content-Type:text/plain; charset="us-ascii"

Marge,
	I have dried sections on slides in a 37C oven to dry them prior to
staining as well as incubated the primary antibody of a IHC procedure at 37C
and never found it to alter the adhesion of the section to the slide
adversely. 	Hope this answers your question.
Regards,

Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov

> ----------
> From: 	Hagerty, Marjorie A.[SMTP:mhagerty@emc.org]
> Sent: 	Tuesday, January 05, 1999 12:20 PM
> To: 	'Histonet'
> Subject: 	antigen retrieval
>
> Hi Everyone,
>
> It was recommended by some list members to use a 37 degree for
> incubating slides treated with certain antibodies prior to
> immunoperoxidase staining. My question is: will slides incubated this
> way stay on during steamer antigen retrieval?
>
> Thanks.
> Warm Regards,
> Marg
> EMC, Rancho Mirage, CA
>




<< Previous Message | Next Message >>