Hello,

TTC is used to assess ischemic damage in experimental models of stroke.
The accuracy of the method been discussed as there are discrepancies
between permanent and transient ischemia of the same duration. We use
TTC staining of fresh brain slices, I mm thick (6 or 12 for the whole
brain) for 30 min., take digital images of them and analyze them with
NIH image, the non stained, pale area is the ischemic area while the
red-orange is the intact tissue. Volume can then be calculated by
integrating the area measurements. For ref. see, among others:

<fontfamily><param>Geneva</param>Bederson, J. B., Pitts, L. H.,
Germano, S. M., Nishimura, M. C., Davis, R. L., and Bartkowski, H. M.
(1986). Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain
for detection and quantification of experimental cerebral infarction in
rats. Stroke <italic>17</italic>, 1304-8.


Park, C. K., Mendelow, A. D., Graham, D. I., McCulloch, J., and
Teasdale, G. M. (1988). Correlation of triphenyltetrazolium chloride
perfusion staining with conventional neurohistology in the detection of
early brain ischaemia. Neuropathol Appl Neurobiol <italic>14</italic>,
289-98.



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________________________________________________________________________________

Peter Siesjö, M.D., Ph.D.

Department of Neurosurgery

University Hospital

S-221 85 Lund, Sweden

Tel: 	+46 46 171274, 2229264

Minicall: 	0740 149001

Fax: 	+46 46 189287, 2224201

Email: 	peter.siesjo@neurokir.lu.se

URL: 	<<http://www.neurokir.lu.se>