Hematoxylin and histoclear

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From:Karen S Pawlowski <kna101@utdallas.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Hi all,

My boss just noticed in the 1985 edition of Cellular Pathology Technique,
Culling, Allison and Barr eds., that food oil derivatives, such as
histoclear, will cause "haematoxylin" staining to rapidly fade.  Is this
true?  I have been having problems with hematoxylin fading for quite some
time and I use histoclear in my paramount all the time. 'Tho I began (8
years ago) by using xylene, and I think those faded just as much.

If histoclear does cause the stain to fade, are there any other
substitutes for xylene that would work?

Thanks in advance for your input.

Karen Pawlowski
Sr Research Assoc./UT Southwestern Med Ctr.
PhD Candidate/ UT Dallas

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