Fluorescence PhotoMicroscopy
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From: | "Patterson, Noelle" <PattersonN@NMRIPO.NMRI.NNMC.NAVY.MIL> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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Content-Type: | text/plain; charset="iso-8859-1" |
I haven't done fluorescence microscopy in years. Now, I have an experiment
I did yesterday that is worth taking photos of. Can anyone give me help on
how to take good fluorescence photomicrographs using an Olympus Vanox? I
don't even remember what film I am supposed to use.
I need to do this pretty soon, the slides are in the refer, in the dark, but
I am not sure how long they will last. I used Vectasheild to mount
coverslips, unfortunately, they it expired in Nov. 1995...I sealed ¾ edges
with nail polish, the 4th was on the bottom of the slide, I will seal that
today...if it is not too messy. What are the bets that the FITC fades by
noon today, when I am finally ready to try to take pictures.
Thanks, in advance, for all your help.
Noelle Patterson
Naval Medical Research Center
Bethesda, Md
pattersonn@NMRIPO.NMRI.NNMC.NAVY.MIL
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