section loss

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From:Terry Hacker <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

Just to add to the current slide and lost section debate, I have been
cutting frozen sections of whole mount ISH stained embryos previously
fixed and post fixed in paraformaldehyde, infiltrated in glucose and
embedded/oriented in OCT. When I come to mount them ( aqueous ) any
attempt to remove the OCT by rinsing in water or PBS results in the
embryos going for a swim! The only way around this is to mount them
"dry" resulting in excessive bubble formation.
I use charged slides, air dry the sections for hours or overnight and
have even tried "fixing" in formalin vapour to stick the sections to
the slide but with no success.
Any ideas?

Terry Hacker,
Medical Research Council,

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