frozen section loss/slide evaluation

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From:Gayle Callis <> (by way of histonet)
To:histonet <>
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I ran two lots of plus charge slides, side by side, with frozen
sections, same results with both lots.  However, sections were air
dried longer, over silica gel, fixed in fresh acetone, air dried and
stored in -80C freezer overnight in sealed box, with bags of silica gel,
in a ziplock baggie containing a large bag of silica gel (beginning
to dislike that blue stuff, somewhat gel'd out!)

Sections were greatly improved, did not have the bubble effect artifact,
but some sections looked strung out, didn't make much difference which
slide lot.
This leads me to some conclusion that our humidity (much higher this
year, due to warmer weather/moist storms) is affecting the sections,
culprit is moisture!  Montana is normally extremely dry in the winter,
this year is an exception, with humidity in the 30's, and I have a cooler
lab since they readjusted the theromstat.  Back to a hot room! and I may
have to store my acetone over anhydrous calcium chloride, decant and use.
Has anyone done this?

I vote for moisture in my case and cannot blame my beloved plus charge

Gayle Callis

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