frozen block storage/-80 freezer

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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When storing any block of tissue at -80C, you need to reseal the block
face with OCT to prevent drying.  We reseal, wrap in foil, put in tubes
(50 ml conical) and can recut tissues stored for years without problems.
And we never sucrose cryoprotect.

See if this helps.

Gayle Callis




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