HI guys,
just a quick question to all you bone buffs out there. I have been
asked to cut cryostat sections of bone/cartilage interfaces. What is
the best way to go about this?. I have tried to do this in the dim and
murky past,  and recall that i had trouble with section adherence.
The cartilage stayed on, but the bone floated free.
Any suggestions? Also  this sound silly, but is it possible to
decalcify bone without fixation? I want to preserve as natural an
architecture, but get rid of the minerals?

Thanks in advance
louise taylor
SAIMR
Johannesburg
SA