Dear all

Does anyone have any experience of using commercial companies to
obtain polyclonal antibodies to novel proteins? What we would like to
do, is to run the partially purified proteins on SDS PAGE, cut bands
out of the gels and then send them off to be injected into a suitable
animal (eg rabbit). Has anyone tried such an approach and does anyone
know of a good company that we could use?

In a similar vein, we have also heard that there are companies that
will do protein sequencing again from bands on gels. Does anyone have
any experience of this?

Many thanks

Mike

Michael Bromley PhD
Histology Department
Paterson Institute
Wilmslow Road
Manchester M20 9BX
UK