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From:"Karen D. Larison" <LARISONK@UONEURO.uoregon.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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A graduate student is interested in determining if there is thyroxine in her
embryonic fish.  She has purchased some anti-T3 and -T4 antibodies and is
wondering if these can be used to detect the thyroxine hormone in sections.
so, what fixation do people use?   I assume the amine-containing thyroxine can
be fixed with an aldehyde, but are some aldehyde-containing fixatives better
than others since this is such a small molecule?  And is antigen retrieval

Thanks so much.  Love the net!

Karen Larison - University of Oregon

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