T3/T4
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From: | "Karen D. Larison" <LARISONK@UONEURO.uoregon.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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Content-Type: | text/plain; charset="us-ascii" |
Histonetters,
A graduate student is interested in determining if there is thyroxine in her
embryonic fish. She has purchased some anti-T3 and -T4 antibodies and is
wondering if these can be used to detect the thyroxine hormone in sections.
If
so, what fixation do people use? I assume the amine-containing thyroxine can
be fixed with an aldehyde, but are some aldehyde-containing fixatives better
than others since this is such a small molecule? And is antigen retrieval
required?
Thanks so much. Love the net!
Karen Larison - University of Oregon
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