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From:"J. A. Kiernan" <> (by way of histonet)
To:histonet <>
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On Wed, 20 Jan 1999, Carla Aiwohi wrote:

> I have a protocol which calls for 1% tartrazine in cellusolve.  Can
>anyone tell
> me what cellusolve is?

  Cellosolve is ethylene glycol monoethyl ether. An alternative name
  is ethoxyethanol. It's in the regular chemical catalogues,

>         Is there anything I can use instead of cellusolove.

  Probably, but you'd need to do quite a lot of experimentation
  and comparison with cellosolve itself.   Tartrazine in cellosolve
  is used in Lendrum's dye displacement technique. It slowly and
  controllably replaces a red anionic dye (originally phloxin)
  until only the desired objects, typically cytoplasmic inclusions,
  remain red. The yellow from the tartrazine can then be kept or
  washed out, as desired. There are many variants, including Bismarck
  brown (which is a cationic dye) instead of tartrazine (anionic),
  but cellosolve is the solvent in all the published methods I've

  The original paper by A. C. Lendrum (J. Path. Bact. 59: 399-404, 1947)
  is a literary masterpiece in the field of staining techniques:
  short, erudite and almost comprehensive. Later work has added little
  to the technical aspects of this method, but the histochemical
  rationale has not been investigated (or perhaps it has and I've
  missed it; something that often happens).

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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