Judy-
Lens is no prolem with routine processing. Soak the block face with a
little water and use Positive charged (silane-coated) slides and everything
should stay on.
Jeff Silverman

----------
> From: rkline@emindustries.com
> To: Judy Trogadis <judy@playfair.utoronto.ca>
> Cc: histonet <HistoNet@Pathology.swmed.edu>
> Subject: Re: lens histology
> Date: Friday, January 15, 1999 4:59 PM
>
> Judy,
>
> I don't have a lot of experience with eyes, but I have processed them
with
> the routine tissues and sectioned them without any problems.  They were
> fixed in 10%NBF.  We did have to use larger base molds.
>
> The paraffin blocks were rough cut and soaked in icy water like the
routine
> tissues.  Did not have a problem with them being brittle.  The
pathologists
> and the chief of opthamology liked the results.
>
> Rande Kline HT (ASCP)
> Technical Services
> EM Science
>
>
>
>
> Judy Trogadis <judy@playfair.utoronto.ca> on 01/15/99 02:40:51 PM
>
> To:   HistoNet@Pathology.swmed.edu (histonet)
> cc:
> Subject:  lens histology
>
>
>
>
> Dear Histoexperts:
>
> We are planning to do immunostaining on human eye lens tissue. Is there
any
> special fixation protocol that would prevent the lens from becoming too
> brittle and difficult to section? I have also heard that the sections can
> detach from slides easily. Is any substrate optimal to prevent this?
> I can already tell that the technical components of this project will be
> tough to work out, however, from past experience, I also know that this
> group has the answer to everything.
>
> Hopefully,
> judy
>
>
> Judy Trogadis
> Eye Research Institute and
> University of Toronto
> Toronto Hospital, Western Div.
> 399 Bathurst  St.
> Toronto, Canada M5T 2S8
>
> phone: 416-603-5088
> Fax:   416-603-5126
> email: judy@playfair.utoronto.ca
>
>
>
>
>
>
>