Supposedly glutaraldehyde crosslinks more than formalin and so tissues
fixed in it should show less immunoreactivity. Even so, electron
microscopists have no problem immuno-labeling their glut-fixed, resin
sections. So I would say it won't cause any problems IF it has not been
fixed too long (will cause same problems as over-fixing in formalin).

Tim Morken, B.S., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

FAX:  (404)639-3043



----Original Message Follows----
Date: Thu, 21 Jan 1999 10:16:50 -0500
From: Scott Stickles <STICKLES@mailbox.hscsyr.edu>
Subject: glutaraldehyde
To: HistoNet@Pathology.swmed.edu

One quick question:

What is your experience in immumoreactivity in glutaraldehyde-fixed
tissue
vs. formaldehyde fixed tissue?

In particular, I want to IHC stain for iNOS and MMP in tissues that were
fixed in glutaraldehyde.

Thanks,
Scott Stickles





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