Aidan,

I always wondered about that too. All I could come up with is that the
concentration is lowered just a bit by the evaporation.

Tim Morken, B.S., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

FAX:  (404)639-3043


----Original Message Follows----
Date: Tue, 26 Jan 1999 11:26:45 +1300
From: "Aidan C. Schurr" <aidan.schurr@stonebow.otago.ac.nz>
Subject: Modified Bielschowsky stain
To: HistoNet <HistoNet@Pathology.swmed.edu>

Hi all,

Have just completed a series of stains using the Modified Bielschowsky
stain for neurofibrillary tangles, with great results.  I was wondering
if
anyone out there in histo-land knows of the significance of the
'evaporated
ammonia' that is called for in the protocol?  Have tried using just
standard conc. ammonia, but results were a bit dubious (may have just
been
the fickle nature of the silver stain).  Not urgent, but any info would
be
appreciated.

Cheers.
Aidan

/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/
	Aidan C. Schurr  BMedLabSc
	   Histology Service Unit		++64 3 4797152
	      Pathology Department
	         Dunedin School of Medicine

	            acschurr@bigfoot.com
   	               aidan.schurr@stonebow.otago.ac.nz
\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\








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