Re: Modified Bielschowsky stain
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From: | "Aidan C. Schurr" <aidan.schurr@stonebow.otago.ac.nz> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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Content-Type: | text/plain; charset="us-ascii" |
>Hello, I am in the process of doing the Bielschowsky stain on mouse brain and
>was wondering if you would share your protocols. The one I have is not
>working or it just may be me. It seems like it has a very short incubation
>time in the silver though (15 minutes)
>
>Thanks for your help
>Cindy
Hi there,
The protocol I was using was basically directly from 'Theory and Practise
of histological Techniques', by Bancroft and Stevens (fourth edition). It
has several different incubations in the silver solutions, for a total of
35 minutes. If you don't have access to this (excellent) book, then email
me, and I will post the protocol here, or send it direct to you :) The
original reference can be found in the journal of Neuropathology and
applied Neurobiology, 1986, 12, 3-9. Happy staining!
Aidan
/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/
Aidan C. Schurr BMedLabSc
Histology Service Unit ++64 3 4797152
Pathology Department
Dunedin School of Medicine
acschurr@bigfoot.com
aidan.schurr@stonebow.otago.ac.nz
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