Re: Lung Embedding Protocol

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From:Katri Tuomala <> (by way of histonet)
To:histonet <>
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sbledsoe wrote:
> I have been asked to Post the following:
> Could someone please suggest a good protocol for embedding lung tissue in
> paraffin.
> Thanks
> Sharon B. Bledsoe
> Indiana University
> School of Medicine
> Dept. of Surgery
> Indianapolis, IN 46202
> sbledsoe@iupui.ed
Dear Sharon,
We don't treat the lung tissue differently from other routine
processing, when dealing with tumors. If the study however involves
other lung diseases, we have a protocol to infuse the fresh specimen
with 10% formalin by a Ghurg(?) method, which I can fax, e-mail, send to
you, if you are interested. This method distends the lung tissue, so
that the alveoli can be studied easier. After a proper fixation time,
the tissue is sectioned and processed normally. Katri.

Katri Tuomala
Anatomic Pathology
Sy.Joseph's Hospital
Hamilton, Ontario, Canada

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