Re: Lost sections

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Another opinion from the peanut gallery:

We do use poly-l-lysine coated slides.  We pick our sections off of the
everyday waterbath that has gelatin added.  We dry our sections at about 75
degrees for 15 minutes.  We use the routine stainer to deparaffinize and
hydrate.  It seems that we do everything that the elite say will not work, yet
our tissue stays on and our stains look great.

The only time we have a wash-off problem is when the tissue has not been fixed
or processed correctly.


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