Re: Golgi question
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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Content-Type: | text/plain; charset="us-ascii" |
On Thu, 21 Jan 1999, Bruce A Rasmussen wrote:
> Does anyone have a suggestion for fixative to follow perfusion with saline
> in the rat that would not confound Golgi staining?
There are several Golgi methods for formalin-fixed brain,
but it's OK (perhaps even better) to go straight into the
Golgi mixture.
A student here has been getting beautiful results with
a method of Gibb & Kolb (1998) J Neurosci Methods 79, 1-4.
It's essentially the Golgi-cox method, but with vibratome
sections instead of celloiding embedding. There's also a
Golgi-Cox variant for frozen sections (Kolb & McClimans
(1986) Stain Technol. 61: 379-380).
John Kiernan
London, Canada.
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