Re: Fixation and embedding of adult murine eyes

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From:"Bill Sinai (Anatomical Pathology)" <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

Date:          Tue, 26 Jan 1999 11:41:55 -0600 (CST)
From:          Carolyn Pressman <>
Subject:       Fixation and embedding of adult murine eyes
To:            HistoNet Server <>

Dear Carolyn,

Many many years ago I worked for a specialist eye pathologist, we
used formaldehyde which contained 1% cetavlon (a type of surface
tension reducer)  I can see no reason why something like this (Tween)
would not do the same thing today.  The fixation was for 24 hours
when we cut a small window in the orb, away from the critical area we
were examining and fixed for another 24 hours.  I thought at the time
the fixation was excessive.  We performed several trichrome type
stains with no apparent ill effects to the staining.

This may prove helpful.


What is the best fixative to use for adult murine eyes?  Does anyone
have any good protocals?  I have found through trial and error that
fixing in neutral buffered formalin only works ok if I fix for 2
days.  I embed in parafin and then section at 7 um.  Most of the
architecture of the eye is intact, but the lens always tears or has
holes in it.  Is there a way to get around this problem?  Is
embedding in plastic a better way to do this? Carolyn Pressman
MD Anderson Cancer Center
University of Texas--Houston

Bill Sinai
Department Manager
Tissue Pathology
ICPMR Westmead Hospital
Phone 61+2+9845 7774  Fax 61+2+9687 2330

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