Re: Daily Digest
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From: | George McNamara <geomcnamara@earthlink.net> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Dear Cindi,
Try VectaShield(R) from
Vector Laboratories
30 Ingold Road
Burlingame, CA 94010
To order, call: (800) 227-6666
For technical assistance:
TEL: (650) 697-3600
FAX: (650) 697-0339
E-mail: vector@vectorlabs.com
http://www.vectorlabs.com (Molecular Biology Apps ->
Fluorescence/VectaShield).
Incidentally, their recommended 25 ul for a 22x22 mm coverglass seems a
bit excessive in terms of potential for excess liquid.
For slippery overglasses, try wicking out excess fluid with a kimwipe.
As for coverglasses, you might try sealing them with any one of:
* vaseline (apply from syringe without needle, but don't get on objective
lens!).
* crazy glue pen (my favorite, let it dry a few minutes before putting on
microscope).
* VALAP (a 1:1:1 solution of vaseline: lanolin:paraffin that is heated to
melt and then painted around the coverglass; make sure it's dry before
putting on scope. I have no idea of the melting temperature or how to stir
them together).
>Date: 18 Jan 1999 10:31:17 -0600
>From: Cindipqr@aol.com
>Subject: Mounting media
>
>Hello Everyone,
> I need some recommendations for a mounting media. It needs to be water
>soluble for 100 um. sections of immunofluorescence. The media I'm using now
>doesn't always dry so the coverslips are moving. This is quite a problem for
>confocal microscopy.
>Thanks in advance,
>Cindi
George McNamara, Ph.D.
Applied Spectral Imaging, Inc.
2120 Las Palmas Drive, Suite D
Carlsbad, CA 92009
voice 760-929-2840 ext 17
fax 760-929-2842
geomcnamara@earthlink.net
http://www.spectral-imaging.com (company)
http://home.earthlink.net/~geomcnamara/Index.html (personal)
Major bio-medical imaging applications:
SKY - Spectral Karyotyping
SPY - Spectral Pathology
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